Adult Stem Cell Expansion Using MCSF and Ozonization
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for expanding adult stem cells from blood are lengthy, invasive, costly, and stressful for patients, with limited therapeutic efficacy due to cell differentiation and handling-related stress, making them unsuitable for widespread clinical use, especially in veterinary medicine.
Innovation Solution
A method involving in vitro treatment of blood samples with Macrophage Colony Stimulating Factor (MCSF) and ozonization to de-program and expand adult stem cells, which can be performed before, during, or after MCSF treatment, using a mixture of O2 and O3 to enhance stem cell growth and de-programming, reducing handling and maintaining stem cell efficacy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If apheresis is used to extract stem cells from blood, then stem cells can be collected for therapy, but the process is lengthy (6-8 hours), painful, and stressful for the patient
Solution Approach 1:
The invention extracts only the necessary component (stem cells) from blood through a simplified procedure using growth factors to mobilize stem cells from bone marrow to peripheral blood, then collects them via a less invasive apheresis process, removing the problematic elements (lengthy duration, patient stress) while retaining the beneficial outcome (stem cell collection)
Solution Approach 2:
The invention performs preliminary action by administering growth factors (G-CSF, GM-CSF, M-CSF, or IL-3) before stem cell collection to mobilize stem cells from bone marrow to peripheral blood, thereby simplifying the subsequent collection process and reducing the time and stress of the actual apheresis procedure
2Quantity of substance
If stem cells are cultivated to obtain adequate numbers for therapy, then sufficient cells are available, but the cells begin to differentiate into other cell types and cannot be qualified and quantified
Solution Approach 1:
The invention maintains continuous maintenance of stem cell undifferentiated state through specific culture conditions (growth factors, culture medium composition) during the expansion process, ensuring that cells remain qualified and quantifiable throughout the cultivation period while achieving sufficient numbers for therapy
3Quantity of substance
If invasive methods are used to collect stem cells from bone marrow or fat, then stem cells can be obtained, but the procedures are invasive (drilling bone or surgery)
Solution Approach 1:
The invention uses growth factors (G-CSF, GM-CSF, M-CSF, or IL-3) as intermediaries to mobilize stem cells from bone marrow to peripheral blood, creating a non-invasive pathway to obtain stem cells that eliminates the need for direct bone marrow drilling or fat tissue surgery
4Duration of action of stationary object
If stem cells are stored through refrigeration in liquid nitrogen, then cells are preserved, but the process requires complex infrastructure and handling
Solution Approach 1:
The invention enables stem cells to be used immediately after collection and minimal processing, eliminating the need for complex long-term storage infrastructure. The cells are utilized in the same session or shortly thereafter, making the system self-sufficient without requiring liquid nitrogen storage facilities
Data Source
Figure 1~2
Figure 3~4
Figure 5~6
AI summary
Method for expanding adult stem cells from blood, comprising the growth and de-programming of the adult blood stem cells of a blood sample which has been taken, using in vitro treatment of the blood sample with MCSF and ozonization of the blood sample.