Stem Cell Filtrate Preparation for Tissue Regeneration

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Solution Overview

Problem

Current cell preparations for hair regeneration and pain alleviation from diseases like osteoarthrosis require cultured stem cells, which can proliferate or differentiate upon transplantation, causing adverse effects and are subject to legal restrictions, while conventional hair restorers have limited effectiveness and durability issues.

Innovation Solution

A stem cell filtrate preparation is developed by removing the cell membrane from adipose-tissue-derived mesenchymal stem cells and adipocytes, eliminating the risk of cell proliferation and differentiation, and combining it with adipocyte components for enhanced therapeutic effects.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If cultured stem cells are used for hair regeneration and pain alleviation, then therapeutic effects are achieved, but cell proliferation and differentiation cause adverse effects and legal restrictions occur

Engineering Contradiction:

Improvetherapeutic effectVSAvoidcell proliferation and differentiation

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent extracts and removes the cell membrane component from stem cells, utilizing only the filtrate (cytoplasm and organelles) while eliminating the membrane that would allow cell proliferation and differentiation. This extraction approach maintains therapeutic effects through secreted factors while removing the harmful capability of cell reproduction.

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent creates a functional copy of stem cell therapy using only the filtrate components (growth factors, cytokines, and other secreted substances) without the actual living stem cells. This copy retains the therapeutic signaling functions while lacking the proliferative and differentiating capabilities of intact stem cells.

Inventive Principle:

Principle #26Copying

2Productivity

If conventional hair restorers are used, then hair growth is promoted, but effectiveness varies among individuals and durability is limited

Engineering Contradiction:

Improvehair growth promotionVSAvoideffect consistency and durability

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

The patent changes the fundamental parameters of the treatment by using stem cell filtrate instead of conventional low molecular compounds or plant extracts. The filtrate contains a complex mixture of bioactive molecules including growth factors and cytokines that provide more consistent and durable effects compared to single-component conventional formulations.

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent employs a composite formulation consisting of multiple bioactive components from stem cell filtrate, including various growth factors, cytokines, and extracellular matrix proteins. This composite approach provides synergistic effects that enhance both the consistency and durability of hair regeneration compared to single-agent conventional treatments.

Inventive Principle:

Principle #40Composite materials

3Quantity of substance

If adipose tissue is cultured to obtain stem cells, then cell preparation is achieved, but cultivation time is extended

Engineering Contradiction:

Improvestem cell preparationVSAvoidcultivation time

Core Design Contradiction:

Quantity of substanceVSLoss of time

Solution Approach 1:

The patent performs preliminary separation and filtration of adipose tissue to obtain stem cell filtrate without requiring full cultivation. The filtrate is prepared directly from the adipose tissue matrix, eliminating the time-consuming culture expansion phase while still obtaining the necessary therapeutic components.

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The patent uses a disposable, non-cultured adipose tissue-based filtrate preparation that eliminates the need for long-term cell maintenance. This approach replaces the time-intensive cultured stem cell system with a ready-to-use filtrate product that provides immediate therapeutic effect without requiring ongoing cultivation.

Inventive Principle:

Principle #27Cheap short-living objects (Disposable)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The stem cell filtrate preparation achieves effective hair regeneration, pain alleviation, and tissue restoration without legal restrictions, offering improved durability and efficacy compared to conventional methods.

Implementation Method 1

removing the cell membrane from adipose-tissue-derived mesenchymal stem cells and adipocytes

Methodology Applied

Scientific EffectCell membrane removal:

Data Source

PatentUS12023356B2Stem cell filtrate preparation and method for preparing same

Publication Date: 2024.07.02 SAEKI MASANORI

AI summary

Provided is a stem cell filtrate preparation useful for hair regeneration, alleviation of pain caused by a disease of an interosseous joint, such as osteoarthrosis or rheumatoid arthritis, and treatment of the disease, and in addition, restoration of a damaged muscle, and prevention or treatment of decubitus. The stem cell filtrate preparation according to one aspect of the present invention is characterized by including a filtrate of a stem cell from which a cell membrane is removed. The stem cell may be at least one selected from at least one selected from an adipose-tissue-derived mesenchymal stem cell, an epithelial stem cell, a pigmented stem cell, an iPS cell, and an ES cell. Furthermore, the stem cell filtrate preparation includes an adipocyte or an effective component extracted from cellular fat.