Stem Cell Migration via Inflammatory Culture Medium

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Solution Overview

Problem

Current methods for enhancing stem cell mobility to inflammatory sites often rely on genetic manipulation, which can lead to side effects such as metastasis and tumor stem cell differentiation, and do not efficiently target inflammatory tissues without unnecessary processes.

Innovation Solution

A method involving culturing stem cells in a culture medium of inflammation-related cells, such as synovial fibroblasts or macrophages stimulated with inflammation-inducing substances, to enhance their migratory ability to inflammatory sites, and a pharmaceutical composition comprising these enhanced stem cells with anti-inflammatory agents, either directly or loaded on nanoparticles.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Speed

If genetic manipulation is used to enhance stem cell mobility, then stem cell mobility to inflammatory sites is improved, but side effects such as metastasis and tumor stem cell differentiation occur

Engineering Contradiction:

Improvestem cell mobilityVSAvoidmetastasis and tumor stem cell differentiation

Core Design Contradiction:

SpeedVSObject-affected harmful factors

Solution Approach 1:

The patent extracts and utilizes the culture medium from inflammation-related cells (such as synovial fibroblasts or macrophages stimulated with inflammation-inducing substances) to enhance stem cell mobility. This approach separates the beneficial migratory signal from the harmful genetic manipulation process, achieving targeted mobility without introducing genetic side effects.

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The culture medium of inflammation-related cells serves as an intermediary substance that transfers the migratory signal to stem cells. Instead of directly genetically modifying stem cells, the patent uses this medium as a mediator to induce expression of chemokine receptors and adhesion molecules, thereby enhancing mobility through a safe indirect pathway.

Inventive Principle:

Principle #24Intermediary (Mediator)

2Reliability

If genetic manipulation is used to enhance stem cell mobility, then targeting ability to inflammatory tissues is improved, but unnecessary genetic processes are introduced

Engineering Contradiction:

Improvetargeting abilityVSAvoidgenetic manipulation process

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent extracts the essential migratory signal from inflammation-related cell culture medium and applies it to stem cells. This eliminates the need for complex genetic manipulation processes while maintaining reliable targeting ability to inflammatory tissues through upregulation of chemokine receptors and adhesion molecules.

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The stem cells are treated with culture medium that naturally contains migratory signals, allowing them to self-regulate and enhance their own targeting ability without external genetic intervention. The cells autonomously respond to the inflammatory signals present in the medium, achieving reliable targeting through their inherent biological mechanisms.

Inventive Principle:

Principle #25Self-service

3Speed

If stem cells are cultured in inflammation-related cell medium, then chemokine receptor and adhesion molecule expression is enhanced, but culture process complexity increases

Engineering Contradiction:

Improvemigratory abilityVSAvoidculture process

Core Design Contradiction:

SpeedVSDevice complexity

Solution Approach 1:

The patent uses culture medium from inflammation-related cells (such as synovial fibroblasts or macrophages) that serves multiple functions: it provides nutrients for stem cell growth, delivers migratory signals, and induces expression of chemokine receptors and adhesion molecules. This multi-functional approach enhances migratory ability without requiring separate complex culture steps.

Inventive Principle:

Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method significantly increases stem cell mobility to inflammatory sites, with enhanced expression of chemokine receptors and adhesion molecules, effectively targeting inflammatory tissues without genetic manipulation, and when combined with anti-inflammatory agents, provides a therapeutic benefit for inflammatory and autoimmune diseases.

Implementation Method 1

chemokines (CCL12) and their receptors (CXCR4)... facilitates MSC delivery to various disease sites

Methodology Applied

Scientific EffectChemokine signaling:

Implementation Method 2

ICAM-cellin interactions, (VACM)-VLA4 interactions, and the extracellular matrix (hereinafter referred to as 'ECM') facilitates MSC delivery

Methodology Applied

Scientific EffectAdhesion: Adhesive

Data Source

PatentUS20220259565A1Method of enhancing mobility of stem cells to inflammatory lesion

Publication Date: 2022.08.18 GACHON UNIV OF IND ACADEMIC COOPERATION FOUND

AI summary

The present invention elates to a novel method for enhancing the performance of stem cells, and more particularly, a method for enhancing the ability of stem cells to migrate to an inflammatory site, comprising culturing the stem cells in a culture medium of inflammation-related cells, and a therapeutic use of the stem cells having enhanced ability to migrate to an inflammatory site for treating inflammatory diseases or autoimmune diseases.