Stem Cell Separation Filter Using Porous Polymer Material
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Solution Overview
Problem
Current methods for separating and recovering stem cells from body fluids and biological tissues are inefficient, often requiring centrifugation, are prone to cell damage, and cannot selectively isolate adherent stem cells without contamination, and lack devices that do not require cell washing procedures.
Innovation Solution
A material for separating stem cells with specific density, fiber diameter, and mesh opening characteristics, made from synthetic polymers like polyesters and polypropylene, used in a filter system that allows for easy recovery of stem cells without centrifugation, using a nonwoven fabric with a container having fluid inlet and outlet ports for washing and cell recovery.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If centrifugation methods are used to separate stem cells, then separation efficiency is improved, but cell damage increases and procedure complexity increases
Solution Approach 1:
The patent replaces mechanical centrifugation with a filter-based physical separation system. The filter material with specific pore sizes and surface properties enables stem cell separation through passive filtration mechanisms, eliminating the need for complex centrifugation equipment and multi-step washing procedures while maintaining high separation efficiency and reducing cell damage.
Solution Approach 2:
The invention employs a filter material with controlled pore structures that selectively capture stem cells based on their size, shape, and surface characteristics. The porous structure allows for efficient separation without requiring centrifugation, directly addressing the contradiction between separation efficiency and procedure complexity.
2Manufacturing precision
If multiple washing steps are performed, then cell purity is improved, but cell damage increases and time consumption increases
Solution Approach 1:
The filter material integrates multiple separation functions into a single component, simultaneously achieving cell capture, washing, and concentration in one operation. This eliminates the need for multiple sequential washing steps while maintaining high cell purity, directly reducing time consumption without sacrificing manufacturing precision.
Solution Approach 2:
The invention merges the functions of cell separation, washing, and concentration into a single filter-based operation. The filter material's unique properties enable it to perform multiple functions simultaneously, reducing both time consumption and cell damage associated with repeated washing steps.
3Ease of operation
If open system procedures are used, then operational flexibility is improved, but contamination risk increases
Solution Approach 1:
The filter system enables self-contained stem cell separation and concentration in a closed environment. The filter material's inherent properties provide selective capture without requiring open manipulations, thereby maintaining operational flexibility while eliminating contamination risks associated with open system procedures.
4Device complexity
If existing filter materials are used, then device simplicity is improved, but stem cell recovery rate decreases
Solution Approach 1:
The patent optimizes critical parameters of the filter material including pore size, fiber diameter, material composition, and surface properties. These parameter changes enable the filter to achieve high stem cell recovery rates while maintaining device simplicity, as the optimized material properties provide selective capture without requiring complex device structures.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enables high-yield, simple, and efficient separation and recovery of stem cells, reducing contamination and cell damage, and allows for the recovery of multipotent stem cells in a closed system, suitable for regenerative medicine applications.
Implementation Method 1
a material for separating stem cell which has a density K of 1.0×104≦K≦1.0×106 and a fiber diameter of 3 to 40 μm
Implementation Method 2
selectively capturing and recovering stem cells from body fluids such as bone marrow fluid, peripheral blood and umbilical cord blood
Implementation Method 3
a filter for separating stem cell which comprises the material for separating stem cell as packed in a container having a fluid inlet port and a fluid outlet port
Data Source
AI summary
The present invention has its object to provide a material for separating stem cell and a filter for separating stem cell, each is capable of selectively separating and recovering, in a simple and easy manner, stem cells from body fluids or biological tissue-derived treated fluids, a method for separating and recovering stem cells, and stem cells obtained by such method. The present invention is a material for separating stem cell which has a density K of 1.0×104≦K≦1.0×106 and a fiber diameter of 3 to 40 μm; a filter for separating stem cell which comprises the material for separating stem cell as packed in a container having a fluid inlet port and a fluid outlet port; a method of separating and recovering stem cells which comprises using the material for separating stem cell or the filter for separating stem cell; and a method of producing a multipotent cell fraction.