Stem Cell Sperm Activator for Conception Rate and Embryo Stability
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Solution Overview
Problem
The challenges in the livestock and medical fields include the variability in embryo collection rates for cattle embryo transfer and lower conception rates with sex-sorted semen, particularly in Holstein dairy cattle, as well as decreased sperm motility due to factors like summer infertility and male infertility conditions such as varicocele.
Innovation Solution
A sperm activator is developed using disrupted cell suspensions from adipose tissue-derived stem cells, dental pulp-derived stem cells, bone marrow-derived stem cells, and umbilical cord blood-derived stem cells, which are easy to prepare and can activate sperm in vitro, enhancing fertilization and conception rates.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If sex-sorted semen is used to produce female calves, then the percentage of female calves born is improved, but the conception rate deteriorates
Solution Approach 1:
The patent introduces adipose tissue-derived stem cells as an intermediary substance that mediates between the sex-sorted semen and the reproductive system. These stem cells are administered alongside or before the sex-sorted semen to activate sperm function and improve conception rates, thereby resolving the contradiction between achieving female calf production and maintaining high conception rates
Solution Approach 2:
The patent changes the physiological parameters of the reproductive system by introducing stem cells that modify the uterine environment, enhance sperm motility, and improve overall fertilization conditions. This parameter change allows sex-sorted semen to achieve conception rates comparable to or exceeding those of conventional semen while maintaining the desired sex ratio
2Quantity of substance
If embryo collection is performed in cattle, then the number of transferable embryos can be obtained, but the collection rate fluctuates greatly
Solution Approach 1:
The patent applies preliminary action by administering adipose tissue-derived stem cells to the donor cows before embryo collection. This preliminary treatment prepares the reproductive system in advance, enhancing embryo development and ensuring more consistent and higher collection rates across different collection events
Solution Approach 2:
The stem cells act as an intermediary that mediates between the donor's reproductive system and the embryo development process, creating a more stable and supportive environment for embryo maturation and collection, thereby reducing fluctuations in collection rates
3Reliability
If sperm motility is improved in disrupted cell suspension, then the conception rate is improved, but the preparation complexity increases
Solution Approach 1:
The patent uses adipose tissue-derived stem cells that can be cultured and prepared in advance, then cryopreserved for long-term storage. These disposable cell preparations can be thawed and administered quickly when needed, simplifying the overall process while maintaining high conception rates. The stem cells are easily obtained from adipose tissue and can be expanded in culture to produce sufficient quantities
Data Source
AI summary
It is an object to provide a technique useful for embryo transfer and development of reproductive medical care. Provided are a sperm activator containing a disrupted product of one or more cells selected from the group consisting of adipose tissue-derived stem cells, dental pulp-derived stem cells, bone marrow-derived stem cells, and umbilical cord blood-derived stem cells as an active ingredient, and an artificial insemination method utilizing the same.