Stereo Cell Observation Device for 3D Density Measurement
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Solution Overview
Problem
Current methods for observing cells in suspension culture vessels lack accurate measurement of cell density due to limitations in imaging and analysis techniques, particularly in three-dimensional positioning and density calculation within specific regions.
Innovation Solution
An observation device equipped with a stereo image-acquisition optical system that captures images of cells floating in culture fluid and an analyzer to identify the three-dimensional position of cells, enabling precise calculation of cell density within predetermined regions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional image acquisition methods are used, then the observation device is simple, but the measurement precision of cell density is insufficient
Solution Approach 1:
The patent transitions from two-dimensional image analysis to three-dimensional spatial analysis by introducing a stereo optical system that captures images at multiple depths. This enables the system to identify cells within a predetermined three-dimensional region, thereby accurately measuring cell density in suspension cultures without requiring complex mechanical structures.
2Measurement precision
If three-dimensional position identification is implemented, then the cell density calculation accuracy improves, but the device complexity increases
Solution Approach 1:
The patent employs a stereo optical system that captures images at multiple depth positions simultaneously, enabling three-dimensional position identification of cells. This dimensional transition allows accurate calculation of cell density within a predetermined three-dimensional region while maintaining relatively simple optical components.
Solution Approach 2:
The patent uses multiple images captured at different depths as copies of the same cellular structure. By analyzing these replicated images and identifying cells across multiple depth planes, the system achieves three-dimensional position identification without requiring complex optical manipulation.
3Measurement precision
If conventional two-dimensional image analysis is used, then the analysis method is simple, but the measurement precision for cell density in suspension culture is insufficient
Solution Approach 1:
The patent extends two-dimensional image analysis to three-dimensional analysis by capturing images at multiple depth positions. This enables the system to detect and measure cell density throughout a predetermined three-dimensional region, overcoming the limitations of conventional two-dimensional methods in suspension culture observation.
Data Source
AI summary
Provided is an observation device including: a stereo image-acquisition optical system that acquires images of cells floating in a culture fluid inside a culture vessel; and an analyzer that calculates a cell density of the cells on the basis of the images acquired by the stereo image-acquisition optical system, wherein the analyzer identifies a three-dimensional position of each of the cells included in the images and calculates the cell density on the basis of the number of cells present within a predetermined three-dimensional region.