Sterile Cell Sampling Device with Integrated Pump and Filter
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Solution Overview
Problem
Existing sterile cell sampling devices are cumbersome and prone to contamination due to frequent opening, which complicates the sampling process and increases the risk of contamination, especially in large volume containers.
Innovation Solution
A quantitative, sterile, and disposable automatic cell sampling device with a sampling container, pump, sterile filter, and control valve, which allows for convenient and controlled sampling by connecting the container to a cell-based drug production device, utilizing a peristaltic pump and sterile filter to minimize contamination risks and optimize space utilization.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If frequent opening of the sampling device cover is performed for cell sampling, then sampling can be conducted regularly to monitor cell indicators, but the risk of contamination increases and the operation becomes troublesome
Solution Approach 1:
The sampling device is divided into separate functional modules: a sampling container with receiving cavity, a pump system, a sterile filter, and control valves. This segmentation allows the sterile sampling container to remain sealed while sampling is performed through dedicated ports, reducing the need to open the main container and thus lowering contamination risk during frequent sampling operations
Solution Approach 2:
A sterile filter is introduced as an intermediary component between the sampling container and the external environment. The filter allows sampling to occur while maintaining sterile separation, enabling frequent sampling without compromising the sterile environment inside the container
2Quantity of substance
If a large volume container is used for cell sampling, then more sample can be collected, but the handling requirements increase and the sterile environment area requirements become more complex
Solution Approach 1:
The sampling device employs a nested structure where the receiving cavity is positioned at the top of the sampling container, maximizing the use of vertical space. The pump, valves, and filters are integrated into a compact arrangement that nests within or around the container, allowing high sampling capacity without proportionally increasing overall device complexity or handling difficulty
Solution Approach 2:
The design utilizes vertical dimension by positioning the receiving cavity at the top of the container and arranging components vertically. This dimensional optimization allows the sampling capacity to be increased by utilizing height rather than expanding horizontal footprint, thereby avoiding increased handling complexity associated with larger horizontal dimensions
3Volume of moving object
If the sampling device is made compact with small volume, then space utilization is improved and handling is simplified, but the sampling capacity may be limited
Solution Approach 1:
The pump system is designed to be dynamically controllable, allowing adjustment of pumping speed and duration to achieve the required sampling capacity. The control valves enable dynamic control of fluid flow paths, allowing the compact device to adapt its sampling rate and total volume collected based on operational requirements, thus achieving high sampling capacity within a small form factor
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device enables sterile and convenient cell sampling with reduced contamination risk, allowing for multiple sampling events while maintaining a small volume and high space efficiency, thus improving the reliability of cell-based drug production monitoring.
Implementation Method 1
cell fluid is introduced into the sampling container by generating a fluid driving force through the pump
Implementation Method 2
the sterile filter is configured to filter air passing therethrough into the sterile air
Implementation Method 3
the control valve is installed in a middle of the first conduit for controlling on and off of the first conduit
Data Source
AI summary
The present disclosure relates to a quantitative, sterile and disposable automatic cell sampling device and method. The automatic cell sampling device includes a sampling container, a pump, a sterile filter, a control valve, a first conduit, and a second conduit. A receiving cavity is formed in an interior of the sampling container, and a first interface and a second interface are formed in a top portion of the sampling container for achieving a maximum sampling capacity of the sampling container. One end of the first conduit is connected to the first interface, and the other end thereof is connected to a cell based drug production device through the pump. The control valve is installed in a middle of the first conduit. The second conduit is connected between the second interface and the sterile filter, and is configured for discharging and supplementing sterile air in the sampling container.