Sterilizable 3D Printer Assembly in Bioreactor Container

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

Current methods for forming three-dimensional structures face challenges in maintaining sterile conditions during transfer from three-dimensional printers to bioreactors or mixing containers, requiring extensive technical effort to prevent contamination.

Innovation Solution

A sterilizable container and method that integrates a three-dimensional printer assembly within a bioreactor or mixing container, allowing for the direct printing and processing of structures under sterile conditions, using materials like glass, stainless steel, or plastic, and enabling the use of biologically active fluids for seeding or preparation without external transfer.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If a three-dimensional structure is printed in a separate three-dimensional printer and then transferred to a bioreactor or mixing container, then the three-dimensional structure can be formed with desired complexity, but the sterile conditions must be maintained during transfer which requires extensive technical effort

Engineering Contradiction:
Improvethree-dimensional structure formation capabilityVSAvoidsterile transfer system complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent merges the three-dimensional printer assembly with the bioreactor or mixing container by providing a printer assembly that is contained within the bioreactor container. This integration eliminates the need for separate printing and bioprocessing equipment, allowing three-dimensional structures to be printed directly within the sterile environment of the bioreactor, thereby avoiding complex sterile transfer requirements while maintaining both printing capability and sterility

Inventive Principle:
Principle #5Merging (Combining)

2Adaptability or versatility

If a three-dimensional structure is printed in a separate three-dimensional printer and then transferred to a bioreactor or mixing container, then the three-dimensional structure can be formed with desired complexity, but the transfer process requires extensive technical effort to maintain sterile conditions

Engineering Contradiction:
Improvethree-dimensional structure formation capabilityVSAvoidtransfer operation simplicity
Core Design Contradiction:
Adaptability or versatilityVSEase of operation

Solution Approach 1:

The patent combines the printing function directly within the bioreactor container through an integrated printer assembly. This merging eliminates the separate transfer operation between printer and bioreactor, allowing structures to be printed in-situ within the sterile environment, thereby dramatically simplifying the operation from complex multi-step transfer procedures to a single integrated printing process

Inventive Principle:
Principle #5Merging (Combining)

3Reliability

If the three-dimensional printer assembly is integrated within the bioreactor container, then sterile conditions are maintained during printing, but the device complexity increases

Engineering Contradiction:
Improvesterile condition maintenanceVSAvoidintegrated system complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent segments the integrated system into distinct functional modules: a printer assembly containing printing components (extruder, print head, build platform) and a bioreactor container containing the sterile environment and biological materials. This modular segmentation allows each component to be optimized independently while maintaining overall system sterility through defined boundaries and controlled interfaces, reducing the complexity burden of integration

Inventive Principle:
Principle #1Segmentation

4Ease of operation

If external transfers are eliminated through integration, then the process of maintaining sterile conditions is simplified, but the device requires integrated design

Engineering Contradiction:
Improvesterile process simplicityVSAvoidintegrated design complexity
Core Design Contradiction:
Ease of operationVSDevice complexity

Solution Approach 1:

The patent divides the integrated system into separable modules (printer assembly and bioreactor container) that can be independently manufactured, sterilized, and assembled. This modular approach simplifies the sterile process by allowing pre-sterilization of components before integration, reducing the complexity of maintaining sterility during operation compared to a fully monolithic integrated design

Inventive Principle:
Principle #1Segmentation

Data Source

PatentEP3194150B1Container for accommodating at least of a least one biologically active fluid and at least one preparatory fluid, and a method therefor
Publication Date: 2020.03.25 SARTORIUS STEDIM BIOTECH GMBH
  • EP3194150B1 patent drawingFigure 1
  • EP3194150B1 patent drawingFigure 2
  • EP3194150B1 patent drawingFigure 3A~3D

AI summary

The present invention refers to a sterilizable container for accommodating at least one of at least one biologically active fluid and at least one preparatory fluid, comprising: - an area within the container configured to accommodate at least one sterilizable three-dimensional printer assembly; - a sterilizable three-dimensional printer assembly including: • at least one printing platform; • at least one printer head for dispensing structural material onto the at least one printing platform in order to form thereon a three-dimensional structure; and • a moving mechanism for providing a relative displacement between the at least one printer head and the at least one printing platform, wherein the area is configured such that the fluid can reach the printer assembly.