Stevia Extraction Solvent Switch Prevents Isochlorogenic Acid Hydrolysis
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Solution Overview
Problem
Traditional Stevia rebaudiana extraction processes lead to hydrolysis of isochlorogenic acid, making it difficult to separate chlorogenic acid from stevioside due to their close polarities, resulting in reduced product quality and efficiency, and increased water consumption.
Innovation Solution
An industrial method involving alcohol extraction followed by adjustment of the feed liquid state using a reagent with pKa < 4.7 to achieve a free molecular state of chlorogenic acid, then using a moderate-polarity water-insoluble organic solvent for extraction separation to enrich chlorogenic acid in an organic layer and stevioside in a water layer, followed by resin adsorption separation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If water extraction is used to extract chlorogenic acid from Stevia rebaudiana, then extraction efficiency is improved, but isochlorogenic acid undergoes hydrolysis resulting in reduced product quality
Solution Approach 1:
The patent changes the extraction parameter from water to alcohol (ethanol or methanol) as the extraction solvent. This parameter change prevents hydrolysis of isochlorogenic acid while maintaining high extraction efficiency, as alcohol extraction does not promote the hydrolytic degradation that occurs with water extraction.
Solution Approach 2:
The patent utilizes the phase transition properties of alcohol by adjusting its concentration and temperature to optimize extraction. The alcohol extract is then concentrated through evaporation to remove the solvent, yielding the purified chlorogenic acid product without hydrolysis.
2Manufacturing precision
If resin separation is used to separate phenolic substances from stevioside, then separation is achieved, but competitive adsorption occurs reducing production efficiency
Solution Approach 1:
The patent extracts chlorogenic acid from the Stevia rebaudiana material using alcohol extraction before the resin separation step. By removing chlorogenic acid in advance, the subsequent resin separation process only needs to handle stevioside, eliminating competitive adsorption and improving production efficiency.
Solution Approach 2:
The patent performs preliminary extraction of chlorogenic acid using alcohol before the main resin separation process. This preliminary action prepares the material by removing interfering substances, allowing the resin separation to proceed more efficiently with fewer complications.
3Quantity of substance
If resin adsorption is performed on both chlorogenic acid and stevioside simultaneously, then comprehensive adsorption occurs, but adsorption capacity for stevioside is reduced
Solution Approach 1:
The patent extracts chlorogenic acid using alcohol extraction before resin adsorption. This removes chlorogenic acid from the mixture, so when resin adsorption is performed, only stevioside remains to be adsorbed, ensuring full adsorption capacity is available for stevioside without competition from chlorogenic acid.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method prevents hydrolysis of isochlorogenic acid, ensures high purity and efficacy of chlorogenic acid, and improves the separation efficiency of stevioside, reducing water consumption and waste, while promoting sustainable production.
Implementation Method 1
carrying out extraction separation with a water-insoluble organic solvent of moderate-polarity to obtain an organic layer enriched with chlorogenic acid and a water layer enriched with stevioside
Implementation Method 2
followed by resin adsorption separation
Data Source
AI summary
Disclosed is an industrial method for simultaneously preparing Stevia rebaudiana chlorogenic acid and stevioside. The industrial method includes carrying out alcohol extraction on Stevia rebaudiana which is used as a raw material, and then adjusting the feed liquid state to allow chlorogenic acid to be in a free molecular state; carrying out extraction separation by a water-insoluble moderate-polarity organic solvent; enriching the Stevia rebaudiana chlorogenic acid in an organic layer; and enriching the stevioside in a water layer. Compared with a traditional water extraction process, the method has the advantages that chlorogenic acid ingredient in the Stevia rebaudiana can be prevented from being hydrolyzed, such that the contents and effects of effective ingredients in Stevia rebaudiana chlorogenic acid products can be guaranteed; effective separation can be carried out on the premise that the quality and the production efficiency of stevioside products are unaffected, the production efficiency can be improved, and the ratio of isochlorogenic acid to total chlorogenic acid in the resulting products is close to that in the raw material; production water consumption can be reduced, and discharge of sewage and flocculation residues can be decreased; and accordingly, the method is a green production process with high benefits.