Stilbenoid Production via Segmented UV Irradiation
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Solution Overview
Problem
There is a need to efficiently and safely increase the amount of stilbenoids, such as resveratrol, and other compounds like TCA cycle metabolites, polyamine alkaloids, 4-aminobutyric acid, abscisic acid, and their salts in plants.
Innovation Solution
The method involves irradiating plants, plant parts, crushed materials, or cultured plant cells with light in the wavelength range of 275-295 nm at a fluence of 50,000-2,500,000 μmol/m2, while ensuring that the fluence at 200-270 nm is less than 20% of that at 275-295 nm, followed by storage in a dark place for 1 day or more.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If plants are irradiated with ultraviolet light (200-270 nm) to increase stilbenoid content, then the amount of stilbenoids increases, but DNA damage and allergen production occur reducing safety
Solution Approach 1:
The patent segments the ultraviolet spectrum into two distinct wavelength ranges: 275-295 nm for beneficial stilbenoid production and 200-270 nm for harmful DNA damage. By treating these wavelength ranges separately and controlling their relative fluences, the method achieves stilbenoid enhancement while avoiding the harmful effects of shorter wavelength UV radiation.
Solution Approach 2:
The patent changes the key parameter of light wavelength from the conventional broad UV range to a specific narrow band (275-295 nm). This parameter change transforms the irradiation process from one that causes DNA damage to one that safely induces stilbenoid biosynthesis, while the fluence ratio parameter (maintaining 200-270 nm fluence at less than 20% of 275-295 nm fluence) further optimizes the safety and efficacy of the treatment.
2Quantity of substance
If conventional UV irradiation methods are used to increase resveratrol content, then resveratrol production increases, but the method lacks safety control and efficiency optimization
Solution Approach 1:
The patent establishes a feedback control mechanism by defining specific fluence ranges (50,000-2,500,000 μmol/m2 at 275-295 nm) and a fluence ratio constraint (200-270 nm fluence less than 20% of 275-295 nm fluence). These parameters provide real-time guidance for irradiation treatment, ensuring that the process remains within safe and effective boundaries, thereby improving reliability and safety control.
Solution Approach 2:
The patent optimizes efficiency by precisely defining the wavelength range (275-295 nm) that maximizes stilbenoid biosynthesis while minimizing harmful effects. This parameter optimization ensures that the irradiation energy is used most effectively for the desired biochemical response, improving both safety and efficiency compared to conventional broad-spectrum UV methods.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method effectively increases the amount of stilbenoids and other target compounds in plants, enhancing their content without causing DNA damage or allergen production, thus improving safety and efficiency.
Implementation Method 1
irradiating the plant, part, crushed material or cultured plant cell with light, wherein a fluence at a wavelength range of 275-295 nm is 50,000-2,500,000 μmol/m2
Data Source
AI summary
The present disclosure relates to a method of increasing an amount of a stilbenoid, and/or one or more compounds selected from the group consisting of TCA cycle metabolites, polyamine alkaloids, 4-aminobutyric acid, abscisic acid and salts thereof in a plant, the method comprising steps of:irradiating the plant, part, crushed material or cultured plant cell with light, wherein a fluence at a wavelength range of 275-295 nm is 50,000-2,500,000 μmol/m2, while at the same time a fluence at a wavelength range of 200-270 nm is less than 20% of the fluence at the wavelength range of 275-295 nm; andstoring the irradiated plant, part, crushed material or cultured plant cell in a dark place for 1 day or more.