Immunochromatography Kit with Cyclic Oligosaccharide for Streptococcus Detection
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Solution Overview
Problem
Current immunochromatography methods for detecting gram-positive bacteria, particularly streptococci, face challenges such as low sensitivity, false positives, and the need for complex nitrous acid preparation, which can lead to errors and inefficiencies in testing.
Innovation Solution
Incorporating a cyclic oligosaccharide and nitrite in the sample extraction solution, which generates nitrous acid on the test device, improving sensitivity and accuracy by preventing protein denaturation and agglutination, and eliminating the need for pre-preparation of nitrous acid.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If nitrous acid is prepared by mixing sodium nitrite and organic acids before extraction, then polysaccharide extraction efficiency is improved, but operation complexity increases and time is lost due to preparation steps
Solution Approach 1:
The patent pre-mixes sodium nitrite and organic acid in a buffer solution to prepare nitrous acid extraction reagent in advance, storing it ready for use. This eliminates the need for operators to mix reagents at the time of extraction, reducing operation complexity while maintaining high polysaccharide extraction efficiency
Solution Approach 2:
The patent divides the extraction system into separate functional components: a buffer solution containing pre-dissolved sodium nitrite and organic acid, and a separate nitrite solution. This segmentation allows flexible combination and simplifies operation by eliminating complex mixing steps while preserving extraction efficiency
2Measurement precision
If nitrous acid is prepared by mixing reagents before extraction, then extraction efficiency is improved, but time is lost due to preparation steps
Solution Approach 1:
The patent prepares nitrous acid extraction reagent by pre-dissolving sodium nitrite and organic acid in buffer solution in advance, storing it ready for immediate use during extraction. This preliminary preparation eliminates time-consuming mixing steps at the time of extraction while maintaining high polysaccharide extraction efficiency
3Measurement precision
If nitrous acid is prepared by mixing reagents before extraction, then extraction efficiency is improved, but reliability decreases due to potential mixing errors
Solution Approach 1:
The patent pre-mixes sodium nitrite and organic acid in buffer solution to prepare standardized nitrous acid extraction reagent in advance, eliminating potential mixing errors during extraction. This preliminary preparation ensures consistent reagent composition and improves diagnosis reliability while maintaining extraction efficiency
Solution Approach 2:
The patent optimizes the buffer solution composition with specific pH (5.0-7.0) and ionic strength parameters to stabilize nitrous acid generation. By controlling these parameters in advance, the patent ensures reliable and reproducible extraction results while maintaining high polysaccharide extraction efficiency
4Ease of operation
If conventional immunochromatography is used for streptococcus detection, then simplicity is maintained, but sensitivity is low and false positives occur
Solution Approach 1:
The patent optimizes buffer solution parameters including pH (5.0-7.0), ionic strength, and composition to enhance antigen-antibody reaction efficiency. These parameter optimizations improve detection sensitivity and reduce false positives while maintaining the simplicity of immunochromatography operation
Solution Approach 2:
The patent uses a composite buffer solution containing multiple components including sodium nitrite, organic acid, and various buffer salts. This composite formulation enhances both the extraction efficiency and the subsequent immunochromatographic detection sensitivity, improving overall test performance while maintaining operational simplicity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enhances test efficiency and accuracy, providing a high signal-to-noise ratio and enabling quick, reliable detection of streptococci without the complications of nitrous acid preparation, thus improving the overall performance of immunochromatographic testing.
Implementation Method 1
improving sensitivity and accuracy by preventing protein denaturation and agglutination
Implementation Method 2
generates a nitrous acid on a test device, instead of preparing a nitrous acid at the time of use for each testing
Data Source
AI summary
Disclosed herein is an immunoassay method for detecting the detection target antigen in an analyte through extraction with an extraction agent. The method is characterized in that the detection is performed in the presence of a cyclic oligosaccharide. The method is also characterized in the use of an immunochromatography kit for detecting gram-positive bacteria in an analyte, and that is configured from an analyte dilution solution, a sample dropping part, an antigen extracting part, a labeling substance retaining part, an immunochromatography medium having a detection part, and an absorption part, and in which an organic acid is retained in the antigen extracting part. The immunochromatography kit contains a cyclic oligosaccharide and a nitrite compound by containing at least one of a cyclic oligosaccharide, a nitrite compound, and a mixture thereof in the analyte dilution solution and/or the sample dropping part.