Streptomyces Fermentation Broth for Rhizosphere Nitrogen Fixation
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Solution Overview
Problem
The application of excessive chemical fertilizers has reduced the nitrogen fixation function of soil microorganisms in agricultural fields, necessitating a solution to enhance the abundance and nitrogen fixation capacity of nitrogen-fixing bacteria in the rhizosphere soil to improve crop yields and reduce fertilizer use.
Innovation Solution
A Streptomyces linyiensis strain, deposited as CGMCC No. 25834, is used to increase the abundance of nitrogen-fixing microorganisms such as Actinomyces, Streptomyces, Rhizobium, Mesorhizobium, and Micromonospora, and enhance nitrogenase activity in the rhizosphere soil, formulated into microbial agents, fertilizers, or pesticides.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If excessive chemical fertilizers are applied, then crop yields are maintained or increased, but the nitrogen fixation function of soil microorganisms is reduced
Solution Approach 1:
The patent uses Streptomyces linyiensis as an intermediary organism to restore nitrogen fixation function in soil. This microorganism produces metabolites that stimulate the activity of indigenous nitrogen-fixing bacteria in the rhizosphere, thereby rebuilding the nitrogen fixation capacity without relying on chemical fertilizers. The Streptomyces acts as a bridge between chemical fertilizer application and natural nitrogen fixation processes.
2Reliability
If chemical fertilizers are reduced, then soil microorganism nitrogen fixation function is improved, but crop yields may decrease
Solution Approach 1:
The patent applies Streptomyces linyiensis in advance to prepare the soil microbial environment before reducing chemical fertilizer application. By pre-establishing the nitrogen fixation capacity through Streptomyces metabolites, the soil can maintain adequate nitrogen supply even when chemical fertilizers are reduced, thus avoiding yield loss while improving nitrogen fixation function.
3Quantity of substance
If Streptomyces linyiensis is applied, then the abundance of nitrogen-fixing microorganisms is increased, but the complexity of microbial community regulation is increased
Solution Approach 1:
The Streptomyces linyiensis application triggers self-service mechanisms in the soil ecosystem. The Streptomyces metabolites naturally stimulate the growth and activity of indigenous nitrogen-fixing bacteria without requiring external intervention or complex management. The microbial community self-regulates and enhances its nitrogen fixation capacity through these natural interactions, simplifying the overall regulation process.
Data Source
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AI summary
Provided are Streptomyces and a use thereof. The Streptomyces is named Streptomyces linyiensis, which was preserved in China General Microbiological Culture Collection Center (CGMCC) on September 28, 2022, and has a preservation number of CGMCC No. 25834. A Streptomyces fermentation broth can be used to significantly improve the abundance of nitrogen-fixing microorganisms in crop rhizosphere soil, improve the nitrogen-fixing enzyme activity of the crop rhizosphere soil, improve the nitrogen-fixing capability of the nitrogen-fixing microorganisms in the crop rhizosphere soil, and promote the absorption of nitrogen nutrients by crops, and can be used for preparing bacterial agents, microbial fertilizers, or biopesticides having corresponding functions.