Streptomyces diastaticus CS1801 Chitinase Production from Prawn Paste
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Solution Overview
Problem
Current methods lack a strain for producing chitinase from prawn paste and utilizing prawns to produce chitooligosaccharides, which have significant anti-neuroinflammation, antioxidant, and anti-tumor effects, limiting their industrial application.
Innovation Solution
The strain Streptomyces diastaticus CS1801, isolated from naturally fermented prawn paste, is used to ferment prawns in a specific medium to produce chitooligosaccharides, with optimal growth conditions at 30°C and pH 6.5, achieving enzyme activity of 57.3 U/L and chitooligosaccharides content of 0.58 mol/L.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If chitosan is decomposed using conventional methods, then chitooligosaccharides can be produced, but the process lacks efficient biological catalysis and high productivity
Solution Approach 1:
The strain Streptomyces diastaticus CS1801 is isolated from naturally fermented prawn paste, utilizing the self-fermentation capability of the microorganism to decompose chitosan into chitooligosaccharides without requiring complex external intervention or artificial fermentation systems
Solution Approach 2:
The fermentation process is optimized by controlling specific parameters including temperature (30-37°C), pH (6.5), and incubation time (5-7 days) to maximize chitinase enzyme activity and chitooligosaccharides yield, achieving enzyme activity of 57.3 U/L and chitooligosaccharides content of 0.58 mol/L
2Adaptability or versatility
If chitosan is decomposed to produce chitooligosaccharides, then water-soluble products with bioactive effects are obtained, but the water insolubility of chitosan limits direct application
Solution Approach 1:
The chitinase enzyme produced by Streptomyces diastaticus CS1801 specifically extracts and decomposes chitosan polymer into smaller water-soluble chitooligosaccharide units, which can then be applied in various fields including food, agriculture, and medicine
Solution Approach 2:
The molecular structure of chitosan is transformed through enzymatic hydrolysis, changing the polymer chain length and molecular weight to produce water-soluble oligosaccharides with enhanced adaptability for various applications while maintaining the bioactive properties
3Reliability
If strains are screened from prawn paste, then a suitable chitinase-producing strain can be obtained, but there is no established method for screening and utilizing such strains
Solution Approach 1:
The naturally fermented prawn paste serves as a ready-made culture medium that supports the growth and chitinase production of Streptomyces diastaticus CS1801, eliminating the need for complex artificial medium preparation and strain cultivation procedures
Solution Approach 2:
The screening process is simplified by optimizing cultivation parameters such as temperature (30°C), pH (6.5-7.0), and incubation time (5-7 days) to enable reliable isolation and identification of high-yield chitinase-producing strains directly from prawn paste samples
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach effectively produces chitooligosaccharides from prawns, leveraging the strain's ability to decompose chitosan into bioactive compounds with broad industrial and medical application prospects, including potential treatments for Alzheimer's disease and tumor activity.
Implementation Method 1
it can be decomposed into water-soluble chitooligosaccharides or chitosan oligosaccharides (COS) under the action of chitinase
Implementation Method 2
The strain Streptomyces diastaticus CS1801, isolated from naturally fermented prawn paste, is used to ferment prawns in a specific medium to produce chitooligosaccharides
Data Source
AI summary
The present invention relates to a strain for producing chitinase and application thereof. The class of the strain is named Streptomyces diastaticus CS1801 and the preservation number thereof is CCTCC NO: M2018263. The Streptomyces diastaticus CS1801 of the present invention is derived from naturally fermented prawn paste. By fermentation of prawns, the enzyme activity of the chitosan is as high as 57.3 U/L and the content of chitooligosaccharides is 0.58 mol/L. The present invention provides a new method for producing chitooligosaccharides and has a good application prospect.