Stromal Progenitor Cell Isolation from Minimal Adipose Tissue
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Solution Overview
Problem
Current methods for obtaining stromal progenitor cells from white adipose tissue are limited by the need for large volumes of tissue, which restricts therapeutic applications to a limited number of patients and requires invasive surgical procedures under general anesthesia, making it unsuitable for individuals with low body mass index and increasing the risk of complications.
Innovation Solution
A method to isolate stromal progenitor cells from a small volume of subcutaneous white adipose tissue using enzymatic or mechanical digestion, followed by culture expansion, allowing for minimal invasive surgery under local anesthesia, enabling the collection of sufficient cells for regenerative therapies from patients of all body types.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If large volumes of white adipose tissue are collected to obtain sufficient stromal progenitor cells, then the number of obtainable cells increases, but the invasiveness of the surgical procedure increases and the risk of complications increases
Solution Approach 1:
The invention changes the parameter of tissue volume required by optimizing the isolation and expansion process. By using specific culture conditions and expansion protocols, the method achieves sufficient cell numbers from smaller tissue volumes, thereby reducing surgical invasiveness and complications while maintaining therapeutic cell quantities
Solution Approach 2:
The method performs preliminary expansion of stromal progenitor cells in vitro before transplantation. This preliminary action allows small initial tissue samples to generate sufficient cell numbers through controlled expansion, eliminating the need to collect large tissue volumes upfront and thereby reducing surgical risk
2Quantity of substance
If large volumes of white adipose tissue are collected to obtain sufficient stromal progenitor cells, then the number of obtainable cells increases, but the procedure requires general anesthesia and becomes unsuitable for patients with low body mass index
Solution Approach 1:
The invention modifies the required tissue volume parameter by implementing efficient expansion protocols. This allows the procedure to be adapted for patients with low body mass index who have limited adipose tissue, expanding the versatility and applicability of the therapy across different patient populations
Solution Approach 2:
The method creates a universal approach that works across different patient types regardless of body mass index. By decoupling the initial tissue collection volume from the final therapeutic cell dose through in vitro expansion, the procedure becomes universally applicable to both thin and obese patients
3Quantity of substance
If prolonged in vitro expansion of stromal progenitor cells is performed to achieve therapeutic doses, then sufficient cell numbers are obtained, but the culture time and process complexity increase
Solution Approach 1:
The method performs preliminary identification and selection of high-proliferative potential stromal progenitor cells before expansion. This preliminary action ensures that only the most proliferative cells are expanded, significantly reducing the culture time required to reach therapeutic doses compared to expanding mixed cell populations
Solution Approach 2:
The invention optimizes culture parameters including media composition, growth factors, and environmental conditions to maximize cell proliferation rate. These parameter changes accelerate the expansion process, reducing the time required to achieve therapeutic cell doses while maintaining cell quality
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method allows for the efficient isolation of stromal progenitor cells from a small amount of adipose tissue, maintaining their immunogenic and differentiative capabilities, and reduces the risks and discomfort associated with traditional fat harvesting, making regenerative therapies accessible to a broader patient population.
Implementation Method 1
isolating adipose stromal progenitor cells from the first quantity q1; putting the first quantity q1 inside a culture media together with a second quantity q2 of a buffer to digest the first quantity and to obtain, after digestion, an expansion of the adipose stromal progenitor cells
Data Source
AI summary
A method of obtaining stromal progenitor cells (SPC) from subcutaneous adipose tissue by incubation of a very small volume of the subcutaneous adipose tissue in an enzyme solution produces SPC that are usable in medical applications based on autologous SPC even on individuals having a body mass index lower than 18.5.