Subtilase Variants for Liquid Detergent Stability and Wash Performance
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing proteases used in liquid detergents face challenges with loss of activity during storage and inadequate wash performance, necessitating improved stability and performance in such formulations.
Innovation Solution
Development of subtilase variants with specific amino acid substitutions, such as R101E, S156D, L262E, and others, enhancing stability and wash performance in liquid detergents.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If existing proteases are used in liquid detergents, then wash performance is achieved, but loss of activity during storage occurs
Solution Approach 1:
The patent applies parameter changes by modifying specific amino acid residues in the protease sequence (e.g., R101E, S156D, L262E substitutions) to alter the enzyme's stability parameters. These sequence modifications enable the protease to maintain structural integrity and catalytic activity in liquid detergent formulations during storage, directly resolving the contradiction between achieving wash performance and preventing activity loss over time
2Reliability
If proteases are modified to improve stability, then storage stability increases, but wash performance may be compromised
Solution Approach 1:
The patent applies local quality by making targeted modifications at specific positions in the protease sequence (such as R101E, S156D, L262E) rather than comprehensive redesign. These localized changes are positioned to affect specific functional regions - with R101E improving storage stability while S156D and L262E maintaining or enhancing wash performance - thereby resolving the contradiction between stability improvement and wash performance maintenance
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The subtilase variants exhibit increased stability and improved wash performance, retaining enzymatic activity over time and effectively removing stains in liquid detergent compositions.
Implementation Method 1
enzymes have for many decades been implemented in washing formulations. Enzymes used in such formulations comprise proteases, lipases, amylases, cellulases, mannosidases as well as other enzymes or mixtures thereof.
Data Source
AI summary
The present invention relates to novel protease variants exhibiting improved stability and or improved wash performance in liquid detergent. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.