Subtilase Polypeptides Enhancing Stability Under Varying pH and Temperature
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current detergent enzymes, particularly proteases, face challenges such as instability due to pH and temperature changes, leading to reduced wash performance and incomplete stain removal in conventional washing conditions.
Innovation Solution
Development of novel polypeptides with specific amino acid sequences (e.g., SEQ ID NO 2, SEQ ID NO 4, SEQ ID NO 6, SEQ ID NO 8, SEQ ID NO 10, SEQ ID NO 12, SEQ ID NO 14, SEQ ID NO 16) that exhibit improved stability and wash performance compared to reference proteases, incorporating alterations at specific positions to enhance their proteolytic activity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional proteases are used in detergent formulations, then basic washing function is provided, but stability under varying pH and temperature conditions deteriorates
Solution Approach 1:
The patent applies parameter changes by modifying amino acid residues at specific positions in the protease sequence (positions 24, 42, 52, 59, 60, 66, 74, 76, 85, 96, 97, 99, 100, 101, 102, 103, 116, 118, 125, 126, 127, 128, 129, 135, 150, 160, 161, 164, 182, 188, 193, 199, 200, 203, 211, 212, 216, 239, 255, 256) to enhance stability under varying pH and temperature conditions while maintaining proteolytic activity
Solution Approach 2:
The patent applies local quality by introducing specific amino acid substitutions at targeted positions within the protease structure, such as replacing residue 24 with aspartic acid or glutamic acid, residue 42 with aspartic acid, glutamic acid, or lysine, to locally improve stability properties without altering the overall enzyme function
2Reliability
If protein engineering is applied to improve enzyme stability, then stability and wash performance are improved, but complexity of enzyme development increases
Solution Approach 1:
The patent applies segmentation by dividing the protease molecule into specific regions of interest (positions 24, 42, 52, 59, 60, 66, 74, 76, 85, 96, 97, 99, 100, 101, 102, 103, 116, 118, 125, 126, 127, 128, 129, 135, 150, 160, 161, 164, 182, 188, 193, 199, 200, 203, 211, 212, 216, 239, 255, 256) and independently optimizing each position through site-directed mutagenesis, thereby systematically improving stability while managing development complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The novel polypeptides demonstrate enhanced stability and wash performance, effectively removing stains like blood under various washing conditions, outperforming traditional proteases in terms of intensity value and Delta remission values.
Implementation Method 1
polypeptides having protease activity... wherein said polypeptide has improved stability and/or improved wash performance compared to a reference protease
Data Source
AI summary
The present invention relates to novel subtilase polypeptides exhibiting increased wash performance and /or improved wash performance and to compositions comprising such polypeptides. The polypeptides of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the polypeptides, expression vectors, host cells, and methods for producing and using the polypeptides of the invention.