Subtilase Variants Enhance Detergent Stability
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Solution Overview
Problem
Current proteases used in detergents face challenges with stability, particularly storage stability, and wash performance under varying temperature and pH conditions, leading to inactivation and reduced effectiveness in removing stains.
Innovation Solution
Development of subtilase variants with specific amino acid substitutions, such as N76D combined with other alterations like Q2R, W6I, and S78D, which enhance stability and wash performance by maintaining enzymatic activity across different conditions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional proteases are used in detergents, then they provide basic proteolytic activity, but they exhibit poor storage stability and inactivate under varying temperature and pH conditions
Solution Approach 1:
The patent applies parameter changes by introducing specific amino acid substitutions (e.g., N76D, Q2R, W6I, S78D) at defined positions in the subtilase protein sequence. These parameter changes in the protein's molecular structure result in improved storage stability and maintained enzymatic activity under varying temperature and pH conditions, directly resolving the contradiction between reliability and duration of action.
2Adaptability or versatility
If proteases are used under varying temperature and pH conditions, then they can handle different washing scenarios, but they become inactivated and lose wash performance
Solution Approach 1:
The patent modifies the protein's parameters through specific amino acid substitutions (N76D, Q2R, W6I, S78D) that enhance the enzyme's ability to maintain its functional state across varying temperature and pH conditions. This allows the protease to adapt to different washing scenarios while maintaining reliable wash performance and stain removal capability.
3Productivity
If existing protease variants are used, then some improvements in activity and solubility are achieved, but further improvement in stability and wash performance is still needed
Solution Approach 1:
The patent introduces additional amino acid substitutions (N76D combined with Q2R, W6I, S78D, and other positions) that build upon existing protease variants to achieve further improvements in both stability and wash performance. These parameter changes enable the enzyme to maintain high productivity while enhancing reliability under diverse washing conditions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The subtilase variants exhibit improved storage stability and wash performance compared to parent proteases, ensuring sustained activity and enhanced stain removal capabilities.
Implementation Method 1
subtilase variants having protease activity
Implementation Method 2
The variants are suitable for use in e.g. cleaning or detergent compositions
Data Source
AI summary
The present invention relates to novel subtilase variants exhibiting increased stability and preferably on par or improved wash performance. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.