Subtilase Variants for Enhanced Detergent Wash Performance
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Solution Overview
Problem
Despite the use of proteases in detergents, there is a need for improved wash performance due to varying washing conditions and the persistence of certain stains under conventional washing conditions.
Innovation Solution
Development of subtilase variants with specific alterations, such as Q59D+Y167A+R170S+A194P+Y209W+L262E, which exhibit improved protease activity and stability, enhancing wash performance compared to the parent subtilase when measured in the AMSA assay.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional proteases are used in detergents, then basic washing function is provided, but wash performance is insufficient under varying washing conditions
Solution Approach 1:
The patent applies parameter changes by modifying multiple amino acid positions in the subtilase sequence simultaneously. The variant comprises specific alterations at positions Q59, Y167, R170, A194, Y209, and L262, which change the physical and chemical properties of the enzyme to improve its activity, stability, and adaptability across different washing conditions including temperature and pH variations.
Solution Approach 2:
The invention creates a composite enzyme structure by combining multiple point mutations within a single protease molecule. This composite approach integrates the effects of six different amino acid substitutions to achieve synergistic improvement in wash performance, thermal stability, and overall reliability that exceeds what single mutations could provide.
2Productivity
If protease activity is increased to improve stain removal, then wash performance improves, but stability under varying conditions may decrease
Solution Approach 1:
The patent simultaneously optimizes multiple parameters of the enzyme including protease activity, thermal stability, and pH stability through coordinated amino acid substitutions. The specific mutations at six positions are designed to work together to enhance both productivity and stability, resolving the trade-off between increased activity and maintained stability.
3Reliability
If multiple amino acid alterations are introduced to improve performance, then wash performance and stability improve, but protein structure complexity increases
Solution Approach 1:
The patent applies local quality by introducing specific amino acid changes at six predetermined positions within the subtilase sequence. Each position (Q59, Y167, R170, A194, Y209, L262) is strategically selected to contribute specific functional improvements while maintaining the overall protein fold and structure. This localized modification approach minimizes structural complexity increases while achieving performance gains.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The subtilase variants demonstrate improved wash performance and stability, effectively removing stains under varying conditions, outperforming the parent subtilase in terms of proteolytic activity and stability in detergent compositions.
Implementation Method 1
the variant has protease activity
Data Source
Figure 1
AI summary
The present invention relates to a detergent composition, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions comprising subtilase variants. The present invention also relates to the use of said detergent compositions in a cleaning process such as laundry or hard surface cleaning. The present invention further relates to a method for removing a stain from a surface, which comprises contacting the surface with the detergent composition.