Subtilisin Variants With Local Amino Acid Changes for Cleaning Stability
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Solution Overview
Problem
There is a need for improved protease variants, particularly subtilisin variants, with enhanced stability and soil removal capabilities for cleaning applications.
Innovation Solution
Development of subtilisin variants with specific amino acid substitutions at position 39 and other positions, such as X003V, X004T, X008V, and others, which enhance stability and cleaning performance.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If subtilisin variants with amino acid substitutions are developed, then stability and soil removal are improved, but protein structure complexity increases
Solution Approach 1:
The patent applies local quality by making specific amino acid substitutions at particular positions (e.g., position 39 and other defined positions) rather than throughout the entire protein. This targeted approach improves stability and soil removal while minimizing overall structural complexity changes, as only specific local regions are modified to achieve the desired performance enhancement.
2Productivity
If subtilisin variants with amino acid substitutions are developed, then cleaning performance is improved, but manufacturing complexity increases
Solution Approach 1:
The patent employs parameter changes by systematically varying amino acid substitutions at specific positions to optimize cleaning performance. By defining specific substitution patterns (e.g., X003V, X004T, X008V), the patent transforms the manufacturing process into a more controlled procedure with defined parameters, making it easier to reproduce high-performance variants while maintaining improved cleaning efficacy.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The variants demonstrate improved cleaning performance and stability, making them suitable for use in laundry and dishwashing compositions, providing effective cleaning solutions.
Implementation Method 1
A protease (also known as a proteinase) is an enzyme that has the ability to break down other proteins. A protease has the ability to conduct proteolysis, which begins protein catabolism by hydrolysis of peptide bonds that link amino acids together
Implementation Method 2
Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin
Data Source
AI summary
Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.