Subtilisin Variant Composition for Stable Soil Removal
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Solution Overview
Problem
There is a need for improved subtilisin variants with enhanced stability for cleaning applications, as existing subtilisins do not meet the required performance standards in terms of stability and soil removal efficacy.
Innovation Solution
Development of subtilisin variants with specific amino acid substitutions at positions 3, 9, 24, 40, 69, 76, 78, 79, 87, 118, 124, 128, 129, 130, 145, 166, 182, 185, 210, 211, 217, 218, 248, and 259, which enhance the stability of the enzyme compared to reference subtilisins.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If existing subtilisins are used for cleaning applications, then basic proteolytic activity is maintained, but stability and soil removal efficacy are insufficient
Solution Approach 1:
The patent applies parameter changes by systematically modifying amino acid residues at 25 specific positions in the subtilisin sequence. Each position offers multiple amino acid substitution options that alter local chemical properties (charge, hydrophobicity, size) to enhance overall enzyme stability while preserving catalytic function for soil removal
Solution Approach 2:
The invention creates composite enzyme variants by combining multiple amino acid substitutions across different regions of the subtilisin molecule. These composite modifications work synergistically to improve both stability and cleaning performance beyond what single mutations could achieve
2Stability of the object's composition
If amino acid substitutions are made to improve stability, then enzyme stability increases, but sequence identity to natural subtilisin decreases
Solution Approach 1:
The patent applies local quality by making targeted amino acid substitutions at 25 specific positions while leaving the rest of the 273-amino acid sequence unchanged. This localized modification approach maintains 70-95% sequence identity to natural subtilisin while introducing stability-enhancing changes at critical positions
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified subtilisin variants exhibit improved stability and soil removal capabilities, making them suitable for use in detergent compositions and cleaning methods.
Implementation Method 1
A protease (also known as a proteinase) is an enzyme that has the ability to break down other proteins. A protease has the ability to conduct proteolysis, by hydrolysis of peptide bonds that link amino acids together in a peptide or polypeptide chain
Data Source
AI summary
Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability compared to one or more reference subtilisin.