Anaerobic Fermentation CO2 Control for Succinic Acid Yield

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Solution Overview

Problem

Current succinic acid production through fermentation, particularly by E. coli, faces low yields and significant carbon dioxide emissions, with traditional methods being inefficient and environmentally undesirable.

Innovation Solution

An anaerobic fermentation process for E. coli, where CO2 supply is controlled by consumption, initially saturating the medium and then discontinuously adjusted, and later consumed to optimize succinic acid production, using a genetically modified strain with specific gene inactivations and a plasmid vector to enhance yield and reduce CO2 emissions.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If traditional fermentation methods are used to produce succinic acid, then succinic acid can be produced, but carbon dioxide emissions are significant and yields are low

Engineering Contradiction:
Improvesuccinic acid yieldVSAvoidcarbon dioxide emissions
Core Design Contradiction:
ProductivityVSObject-generated harmful factors

Solution Approach 1:

The patent changes the physical-chemical parameters of the fermentation process by controlling CO2 supply dynamics. Specifically, it transitions from continuous CO2 supply to a controlled regime where CO2 supply is adjusted based on consumption rates, and implements a two-stage fermentation process with different CO2 supply conditions, thereby improving succinic acid yield while reducing CO2 emissions

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The fermentation process is designed to be self-regulating regarding CO2 supply. The system uses the microorganism's own CO2 consumption rate as feedback to control CO2 supply, eliminating the need for external CO2 addition and preventing excess CO2 emissions while maintaining optimal fermentation conditions

Inventive Principle:
Principle #25Self-service

2Productivity

If CO2 is continuously supplied during fermentation, then succinic acid production is maintained, but residual CO2 concentrations remain high and emissions increase

Engineering Contradiction:
Improvesuccinic acid production rateVSAvoidresidual CO2 concentration
Core Design Contradiction:
ProductivityVSQuantity of substance

Solution Approach 1:

The patent implements a feedback control mechanism where CO2 supply is dynamically adjusted based on the microorganism's consumption rate. The system monitors CO2 consumption and adjusts supply accordingly, and in the second fermentation stage, completely stops CO2 supply to allow consumption of residual CO2, thereby reducing residual CO2 concentration while maintaining production efficiency

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The fermentation process is divided into two distinct stages with different CO2 supply strategies. The first stage uses controlled CO2 supply based on consumption, while the second stage stops CO2 supply entirely to consume residual CO2. This segmentation allows optimization of both succinic acid production and CO2 emission reduction

Inventive Principle:
Principle #1Segmentation

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This process maintains succinic acid production yields while significantly reducing carbon dioxide emissions and residual CO2 concentrations, making the method more environmentally friendly and industrially viable.

Implementation Method 1

process for producing succinic acid and/or succinate ions by fermentation under anaerobic conditions

Methodology Applied
Scientific EffectFermentation: Fermentation

Implementation Method 2

supply of CO2 is controlled by the consumption of CO2 by the strain

Methodology Applied
Scientific EffectCarbon dioxide consumption: Absorption (physical)

Data Source

PatentEP2265723B1Methods for producing succinic acid
Publication Date: 2012.07.18 ROQUETTE FRERES SA

AI summary

The invention relates to methods for producing succinic acid and/or succinate ions by fermentation under anaerobic conditions.