Bv_CSM63713 Sugar Beet Event for Stable Multi-Herbicide Tolerance
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Solution Overview
Problem
Existing transgenic sugar beet events face variability in transgene expression and integration, leading to unpredictable performance and phenotypic differences, complicating the development of commercially viable multi-gene herbicide tolerance traits.
Innovation Solution
The development of recombinant DNA molecules, specifically targeting the sugar beet event Bv_CSM63713, which includes multiple herbicide tolerance genes integrated at a single genomic location, along with methods for precise detection and characterization of these events, ensuring stable and consistent expression.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If multiple herbicide tolerance genes are integrated into sugar beet plants through conventional transformation methods, then herbicide tolerance traits are achieved, but transgene expression variability and integration differences cause unpredictable performance and phenotypic differences
Solution Approach 1:
The patent combines multiple herbicide tolerance genes (glyphosate tolerance EPSPS, dicamba tolerance DMO, and glufosinate tolerance PAT) into a single integrated transgenic construct that is inserted at one specific genomic location. This merging of multiple traits into a single integration event eliminates the variability and complexity that would arise from multiple separate integrations, while maintaining stable and predictable expression of all three herbicide tolerance traits throughout the plant and its progeny.
2Adaptability or versatility
If multiple herbicide tolerance traits are combined through crossing and selection, then weed control options increase, but breeding complexity and cost increase
Solution Approach 1:
The patent segments the complex task of combining multiple herbicide tolerance traits into a single, pre-engineered transgenic construct that contains all three genes (EPSPS, DMO, and PAT) in one integrated unit. This segmentation of the transformation process allows the entire multi-trait combination to be achieved in a single transformation and selection event, rather than requiring multiple separate breeding cycles to combine different traits from different parents.
3Productivity
If transgenes are inserted at random locations in the genome, then transformation efficiency increases, but expression consistency and predictability decrease
Solution Approach 1:
The patent applies local quality by targeting the insertion of the multi-gene construct to a specific genomic location (chromosome 4 in sugar beet) rather than allowing random integration. This localized insertion strategy ensures that the transgenes are integrated at a consistent position across all transformants, leading to uniform expression patterns and predictable phenotypic outcomes, while the transformation method itself maintains high efficiency through optimized Agrobacterium-mediated transformation protocols.
Data Source
AI summary
A transgenic sugar beet event, Bv_CSM63713, is provided. Transgenic plant cells, plant parts, plants, seeds, agricultural and commodity products containing event Bv_CSM63713 are also provided. Recombinant DNA molecules unique to the event Bv_CSM63713, and methods of using and detecting Bv_CSM63713 are also provided. Sugar beet plants containing the event Bv_CSM63713 exhibit tolerance to benzoic acid auxins such as dicamba; inhibitors of EPSPS such as glyphosate; and inhibitors of glutamine synthetase such as glufosinate.