Sulfamoyl-Pyrene Fluorescent Dye for pH Sensor
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Solution Overview
Problem
Conventional optical pH sensors are limited by 'leaching' issues, making them single-use, and are sensitive to ionic strength variations, which affects measurement accuracy, particularly in biotechnological applications requiring a broader pH range and stability across varying ionic conditions.
Innovation Solution
A fluorescent dye with a specific formula, including a pyrene basic structure and sulfamoyl groups, is developed, which allows for extended pH measurement range without additional auxiliary dyes and is less sensitive to ionic strength changes, enabling robust and cost-effective optical pH sensing. This dye is immobilized in an analyte-permeable matrix and used in an optical pH sensor system.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If HPTS or DHPDS dyes are used in conventional optical pH sensors, then the sensor can measure pH in the range of 6-9, but the sensor cannot measure pH in the range of 4-8 and requires additional auxiliary dyes to extend the measurement range
Solution Approach 1:
The patent modifies the chemical structure of the fluorescent dye by introducing a sulfamoyl group at the 1-position of the pyrene nucleus, which shifts the pH measurement range to 4-8. This structural parameter change allows the sensor to measure pH in the desired range without requiring additional auxiliary dyes, thus extending adaptability while maintaining simplicity
Solution Approach 2:
The new fluorescent dye with sulfamoyl group serves multiple functions: it provides pH measurement capability in the 4-8 range, maintains fluorescence properties suitable for optical detection, and eliminates the need for auxiliary dyes. This multi-functionality resolves the contradiction by achieving extended measurement range through a single dye molecule rather than multiple components
2Reliability
If conventional optical pH sensors are used, then pH measurement is possible, but the sensors suffer from 'leaching' issues and can only be used once
Solution Approach 1:
The patent employs a composite material system consisting of the sulfamoyl-pyrene fluorescent dye immobilized in a polymer matrix (such as polyacrylamide or polyvinyl alcohol). This composite structure prevents dye leaching while maintaining pH sensing functionality, enabling the sensor to be reused multiple times and thus improving reliability without substance loss
3Measurement precision
If HPTS or DHPDS-based sensors are used, then pH measurement is possible, but the measurement is heavily influenced by ionic strength variations in the medium
Solution Approach 1:
The introduction of the sulfamoyl group at the 1-position of the pyrene nucleus changes the electronic and steric parameters of the fluorescent dye, making it less sensitive to ionic strength variations. This parameter change in the dye structure reduces the harmful effect of ionic strength on measurement precision while maintaining accurate pH determination in the 4-8 range
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The new fluorescent dye provides an extended measurement range shifted to more acidic pH values, is resistant to ionic strength variations, and prevents 'leaching', resulting in precise and reliable pH determination suitable for various biotechnological applications.
Implementation Method 1
fluorescence-based optical pH sensors have been increasingly described, where a fluorescent dye acts as 'indicator'. Depending on the pH, such fluorescent dyes have a change in the excitation or emission wavelength. If, for example, the fluorescent dye has two different excitation wavelengths, then the pH can be determined from the quotient of the intensities measured in succession at the two excitation wavelengths
Data Source
AI summary
The present invention relates to a new type of fluorescent with the following formula (I), its preparation process, and also an optical pH sensor which comprises this fluorescent dye immobilized on an analyte-permeable carrier.