Supramolecular Receptor for Reversible HS- Detection
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Solution Overview
Problem
Current methods for detecting hydrogen sulfide (H2S) and its anionic form (HS−) in biological systems are plagued by irreversibility, which hinders real-time monitoring of biological processes and disrupts endogenous concentrations, necessitating a reversible detection approach.
Innovation Solution
Development of supramolecular receptor compounds that utilize reversible, non-covalent binding interactions, specifically hydrogen bond donors and acceptors, to selectively and reversibly bind HS−, allowing for accurate detection without altering analyte concentrations, featuring association constants as high as 90,300 M−1.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If current detection methods are used, then detection capability is achieved, but irreversibility occurs which disrupts endogenous concentrations and hinders real-time monitoring
Solution Approach 1:
The patent changes the chemical binding parameter from irreversible covalent bonding to reversible non-covalent interactions. The receptor uses hydrogen bonding and electrostatic interactions with dissociation constants (Kd) in the micromolar to nanomolar range, allowing reversible binding that maintains physiological H2S/HS− concentrations while enabling continuous detection.
Solution Approach 2:
The patent introduces a supramolecular receptor as an intermediary that mediates the detection of H2S/HS− through reversible binding. This receptor acts as a bridge between the analyte and the detection system, using hydrogen bond donors and acceptors to create reversible complexes that report analyte presence without irreversible consumption.
2Duration of action of moving object
If reversible binding is implemented, then real-time monitoring is enabled and endogenous concentrations are preserved, but binding strength must be optimized
Solution Approach 1:
The patent optimizes the binding parameter by tuning the dissociation constant (Kd) to specific ranges (micromolar to nanomolar). This parameter optimization ensures sufficient binding strength for reliable detection while maintaining reversibility for real-time monitoring. The receptor structure incorporates multiple hydrogen bond donors and acceptors to achieve the desired Kd range.
Solution Approach 2:
The patent employs composite molecular structures combining multiple functional groups (hydrogen bond donors, acceptors, and aromatic moieties) within the receptor. This composite approach at the molecular level creates synergistic interactions that provide both sufficient binding affinity and reversible character, enabling real-time monitoring with reliable signal generation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables real-time, reversible detection of HS− without disrupting biological processes, providing selective and strong binding, and informing new detection strategies that avoid irreversible covalent modification, thereby advancing the understanding of H2S roles in biological systems.
Implementation Method 1
supramolecular receptor compounds that utilize reversible, non-covalent binding interactions, specifically hydrogen bond donors and acceptors, to selectively and reversibly bind HS−
Data Source
AI summary
A device that includes a polymeric layer, wherein the polymeric layer comprises (A) a polymer and, incorporated within the polymeric layer, (B) a compound, or a protonate or salt thereof, wherein the compound, or a protonate or salt thereof, has a structure that includes at least one moiety configured for reversible, non-covalent binding of the anionic sulfide species.