Surface-Immobilized Macromolecules for Low-Abundance Biomolecule Capture
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Solution Overview
Problem
Current methods for capturing low-abundance biomolecules in proteomics are inadequate, necessitating improved compositions and structures for effective detection.
Innovation Solution
Development of macromolecules with specific recurring units, such as Formula (I), (II), and (III), which include various functional groups like alkyl, aryl, heteroaryl, and disulfides, to enhance the capture and detection of low-abundance biomolecules.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional capture compositions are used, then the detection of low-abundance biomolecules is attempted, but the sensitivity and specificity are insufficient
Solution Approach 1:
The patent employs composite macromolecules comprising multiple distinct functional groups (e.g., hydrophobic groups, hydrogen-bonding groups, electrostatic groups) within a single capture composition. This composite structure enables simultaneous interaction with multiple aspects of the low-abundance biomolecule, enhancing both sensitivity through stronger binding and specificity through multi-point recognition that reduces false positives.
Solution Approach 2:
The capture compositions are designed with spatially distributed functional groups that provide different local binding characteristics. Hydrophobic regions, polar regions, and charged regions are positioned throughout the macromolecule structure, allowing different portions of the biomolecule to be captured through locally optimized interactions, thereby improving overall detection precision.
2Measurement precision
If capture compositions are designed for high sensitivity, then low-abundance biomolecules can be detected, but the complexity of the composition increases
Solution Approach 1:
The macromolecular capture compositions are designed to perform multiple functions simultaneously: hydrophobic interaction, hydrogen bonding, and electrostatic attraction. This multi-functionality allows a single composition to achieve high sensitivity through diverse binding mechanisms without requiring multiple separate compositions or complex multi-step procedures.
Solution Approach 2:
The patent merges multiple functional groups that would traditionally require separate capture agents into a single integrated macromolecule. By combining hydrophobic, polar, and charged functional groups within one molecular structure, the composition achieves enhanced sensitivity while simplifying the overall capture system architecture.
Data Source
AI summary
Disclosed herein are macromolecules, compositions thereof, and methods of making the same. In one aspect, the macromolecule are immobilized to a surface. In one aspect, provided herein are compositions, systems and kits comprising the macromolecules.