Surface-Modified Culture Plate for Simpler Cell Seeding
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Solution Overview
Problem
Conventional cell culture devices require cumbersome operations such as coating, drying, and washing of the culture surface to attach adherent cells, which hampers efficiency and increases operational complexity.
Innovation Solution
A cell culture device with a culture plate having a surface-modified nanofiber membrane or plasma-treated film that allows direct adherence of cells, combined with a body and cover member fixed via fusion mounts, eliminating the need for surface modification and washing steps.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a coating solution is applied to modify the culture surface and then washed, then cells can adhere smoothly to the culture surface, but the seeding operation becomes very cumbersome
Solution Approach 1:
The culture plate is pre-coated with coating solution and dried before use, so that when the user receives the culture plate, the culture surface is already prepared for cell adhesion. This preliminary preparation eliminates the need for users to perform coating and washing operations, making the seeding operation simple while ensuring reliable cell adhesion
Solution Approach 2:
The culture plate is designed as a disposable single-use product. The coating is applied during manufacturing, and the entire plate is discarded after one use. This approach eliminates the need for users to perform complex coating and washing procedures, as the plate comes pre-prepared and requires no cleanup, thus simplifying operation while ensuring consistent cell adhesion quality
2Reliability
If a coating solution is applied to modify the culture surface, then cells can adhere smoothly to the culture surface, but the production cost increases
Solution Approach 1:
The coating application and plate manufacturing processes are merged into a single integrated production line. The coating solution is applied automatically during plate fabrication, and the drying process is integrated into the manufacturing workflow. This consolidation eliminates separate coating and washing operations, reducing labor and material costs while maintaining consistent coating quality for reliable cell adhesion
Solution Approach 2:
The coating is applied and dried during the plate manufacturing process itself, before the plate reaches the user. This preliminary preparation during production eliminates the need for post-manufacturing coating operations and quality control steps, reducing overall production costs while ensuring every plate leaves the factory with a ready-to-use culture surface that guarantees reliable cell adhesion
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Facilitates seamless cell seeding while maintaining mass productivity and reducing operational complexity and cost.
Implementation Method 1
the surface of the culture plate forming the culture surface is modified so that the cells can smoothly adhere to the culture surface
Implementation Method 2
a support member attached to one surface of the nanofiber membrane via an adhesive layer so as to support the nanofiber membrane
Implementation Method 3
the support member may include a fusion surface formed on one surface to surround an edge of the nanofiber membrane, wherein the fusion surface may be in direct contact with the body and fixed thereto
Implementation Method 4
the body may include at least one fusion mount protruding outward at a certain height along a lower edge thereof, wherein the fusion mount may be fused with the fusion surface of the support member to fix the culture plate to the body
Implementation Method 5
the body may include at least one fusion mount protruding outward at a certain height along the upper edge thereof, wherein the cover member may be fused to one surface of the body through the fusion mount
Implementation Method 6
the culture plate may further include a release film laminated on one surface thereof
Data Source
AI summary
A cell culture device is provided. A cell culture device according to an exemplary embodiment of the present invention includes: a body including a culture space having an upper surface and lower surface that are open; a planar culture plate that is fixed to the body so as to cover the open lower surface of the culture space and forms a culture surface on which cells are cultured, the surface forming the culture surface being modified to allow the cells to easily attach to the culture surface; and a cover member that is fixed to the body so as to cover the open upper surface of the culture space.