Sw-5b2 Nucleotide Sequence Resists TSWV Resistance-Breaking Strains
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Solution Overview
Problem
Tomato plants are vulnerable to 'resistance-breaking' strains of the Tomato Spotted Wilt Virus (TSWV), which can infect plants with the Sw-5b allele, and existing resistance mechanisms are not effective against these mutant strains, necessitating a new means of conferring resistance.
Innovation Solution
A new nucleotide sequence, Sw-5b2, has been identified, providing moderate resistance to TSWV 'resistance-breaking' strains while maintaining high resistance to wild-type TSWV strains, and can be used for marker-assisted selection and introgression into tomato plants.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If the Sw-5b allele is used for resistance to TSWV, then high resistance to wild-type TSWV strains is achieved, but vulnerability to resistance-breaking strains occurs
Solution Approach 1:
The patent applies parameter changes by modifying the nucleotide sequence at specific positions (1054, 1072, 1096, 1116, 1136) within the Sw-5b gene to create new alleles (Sw-5b3, Sw-5b4, Sw-5b5, Sw-5b6) that maintain resistance to wild-type TSWV while gaining resistance to resistance-breaking strains. This involves changing specific nucleotide parameters (A/T/G/C at defined positions) to alter the protein's resistance profile without losing its fundamental function.
Solution Approach 2:
The patent creates composite resistance mechanisms by combining elements from different Sw-5b alleles. The new alleles represent composite solutions that integrate resistance properties from multiple sources (Sw-5b, Sw-5b2, and resistance-breaking strain exposure) to produce a gene variant with broader resistance spectrum, analogous to creating composite materials with enhanced properties.
2Adaptability or versatility
If new Sw-5b alleles are developed for broader resistance, then resistance to resistance-breaking strains is improved, but genetic complexity increases
Solution Approach 1:
The patent applies segmentation by dividing the Sw-5b gene into discrete variable positions (1054, 1072, 1096, 1116, 1136) that can be independently modified. Each position represents a segment that can be changed to confer resistance to specific resistance-breaking strains, allowing systematic development of new alleles without redesigning the entire gene structure.
Solution Approach 2:
The patent implements local quality by making targeted nucleotide changes at specific positions within the Sw-5b gene rather than altering the entire sequence. Each positional change (e.g., position 1054: A/T/G/C) confers specific resistance properties while maintaining the overall gene structure and function, allowing precise tailoring of resistance characteristics.
Data Source
AI summary
The present invention is directed to an isolated nucleotide sequence comprising SEQ. ID. No: 1, or comprising complementary sequences thereof. This invention also regards the use of a nucleotide sequence comprising SEQ. ID. No: 1, or comprising a complementary sequence thereof, for marker assisted selection of plants. In addition, the invention regards the use of a nucleotide sequence comprising SEQ. ID. No: 1, or comprising a complementary sequence thereof, for introgression into plants. Furthermore, the invention is directed to a method to make plants resistant to infections by TSWV strains harmful to plants having the Sw-5b allele, by introducing into the plant genome a nucleotide sequence comprising SEQ. ID. No: 1, or comprising a complementary sequence thereof.