Sweet Corn Line SHY-6S21-6018LL for Uniform Trait Breeding
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Solution Overview
Problem
Existing corn breeding methods struggle to develop uniform varieties with desirable traits such as herbicide tolerance, insect resistance, and disease resistance, as they often result in unpredictable genetic diversity and non-uniform populations.
Innovation Solution
Development of the corn line SHY-6S21-6018LL, which incorporates a single locus conversion through genetic engineering techniques like CRISPR/Cas systems, allowing for precise introduction of traits like herbicide tolerance, insect resistance, and disease resistance, and the production of a homogeneous seed population.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If traditional breeding methods (pedigree breeding and recurrent selection) are used to develop inbred plants, then genetic diversity is increased through combining backgrounds from multiple plants, but the resulting populations are non-uniform and performance is unpredictable
Solution Approach 1:
The patent segments the breeding process into distinct phases: developing uniform inbred lines through self-pollination and selection, then crossing specific inbreds to produce F1 hybrids. This segmentation allows genetic diversity to be captured in the inbred development phase while ensuring uniformity in the final hybrid product, resolving the contradiction between genetic diversity and performance predictability
Solution Approach 2:
The patent changes the genetic uniformity parameter by using multiple generations of self-pollination (S1, S2, S3, S4, S5) to increase homozygosity. This parameter change transforms genetically diverse breeding populations into uniform inbred lines, which then produce consistent F1 hybrids, thereby achieving both genetic diversity in the breeding pool and predictability in the final variety
2Reliability
If self-pollination and selection over many generations are used to develop homozygous inbred plants, then uniform populations are produced, but the process requires many generations and time
Solution Approach 1:
The patent performs preliminary action by developing and maintaining a library of pre-characterized inbred lines (SHY-6S21-6018LL and related lines) with known genetic properties. This preliminary development of uniform inbreds allows future hybrid varieties to be created quickly by crossing pre-selected lines, rather than starting from scratch each time, thus reducing breeding duration while maintaining uniformity
3Reliability
If crossing two homozygous inbred plants is used to produce uniform F1 hybrid population, then all progeny are heterozygous and uniform, but the parental inbreds must be developed first requiring multiple generations of selfing
Solution Approach 1:
The patent uses molecular markers to copy or replicate the identification of desirable genetic traits across different inbred lines. By using marker-assisted selection, the same uniformity standards can be replicated across multiple inbred line developments, ensuring consistent F1 hybrid uniformity without having to re-develop entire breeding programs, thus simplifying the overall process complexity
Data Source
AI summary
The invention provides seed and plants of corn line SHY-6S21-6018LL. The invention thus relates to the plants, seeds, and tissue cultures of corn line SHY-6S21-6018LL and to methods for producing a corn plant produced by crossing such plants with themselves or with another corn plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.