SWIR Fluorescence Imaging with Synchronized Multicolor Excitation
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Solution Overview
Problem
Existing SWIR imaging systems lack the capability for real-time multicolor imaging of biological structures due to limitations in high-throughput detectors and synchronized excitation sources, leading to sub-standard results and limited dynamic range, especially in biological tissues.
Innovation Solution
Employing high-power excitation sources combined with state-of-the-art InGaAs SWIR detectors and SWIR-illuminated fluorophores, synchronized to illuminate and detect fluorescent polypeptides in the NIR and SWIR ranges, enabling multi-color imaging with high dynamic range and faster frame rates.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If existing SWIR imaging systems are used, then imaging is possible, but real-time multicolor imaging capability is limited due to lack of integration of high-power light sources with SWIR detectors
Solution Approach 1:
The patent combines multiple high-power light sources (VIS, NIR, and SWIR lasers) with SWIR detectors in a single integrated imaging system. This merging enables simultaneous excitation of multiple fluorophores with different excitation wavelengths and detection of their emissions in the SWIR range, achieving real-time multicolor imaging capability that was previously unavailable.
Solution Approach 2:
The imaging system is designed to handle multiple excitation wavelengths (VIS, NIR, SWIR) and detect multiple emission wavelengths simultaneously using a single SWIR detector. This multi-functional design allows the system to perform various imaging modes (single-color, multicolor, real-time) without requiring separate specialized equipment for each mode.
2Measurement precision
If visible or near-infrared spectrum imaging is used, then imaging is possible, but resolution and contrast are reduced due to higher photon scattering in biological tissues
Solution Approach 1:
The patent changes the detection wavelength parameter from visible/NIR to SWIR range (1000-2500 nm). This parameter change exploits the optical window in biological tissues where photon scattering is minimized, thereby improving imaging resolution and contrast. The system uses fluorophores that emit in the SWIR range, allowing detection at wavelengths that penetrate biological tissues more effectively.
3Illumination intensity
If high-power light sources are used, then excitation intensity is sufficient, but safety concerns arise as they are safety critical apparatus
Solution Approach 1:
The patent introduces safety interlocks and control systems as intermediary components between the high-power light sources and the users/environment. These safety mechanisms monitor and control the operation of high-power lasers, ensuring that excitation intensity is sufficient for imaging while preventing hazardous exposure. The system includes interlocked enclosures and control systems that manage the safe operation of high-power VIS/NIR/SWIR light sources.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Achieves real-time multi-color imaging with improved resolution, contrast, and penetration depth, overcoming the limitations of existing systems by utilizing existing fluorescent proteins optimized for SWIR emission.
Implementation Method 1
The principle concept of fluorescence imaging requires a (labelled) fluorescent biological sample, an optical setup (microscopic, mesoscopic or macroscopic) for detection and an excitation light source
Implementation Method 2
state of the art InGaAs SWIR detectors
Data Source
AI summary
The present invention relates to systems, methods and fluorescent polypeptide for real-time multicolor shortwave infrared fluorescence imaging. The systems and methods of the present invention further relate to real-time multi-color in vivo SWIR imaging systems employing high-power excitation sources in combination with state of the art InGaAs SWIR detectors and SWIR illuminated fluorescent polypeptide.