Synbiotic Composition with Enzyme-Produced Prebiotics for Strain Selectivity
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Solution Overview
Problem
There is a lack of a selective prebiotic for Lactobacilli probiotic strains, which hinders the specific growth and health benefits of these bacteria in the gut, and existing synbiotics do not effectively target specific probiotic species for enhanced health benefits.
Innovation Solution
A synbiotic composition is developed comprising a probiotic bacterial strain capable of producing a specific prebiotic growth medium through reverse enzyme reactions, using saccharolytic or glycosidase enzymes, which acts as a selective growth medium for the probiotic, promoting its growth over other strains, with preferred components like galacto-oligosaccharides and fructo-oligosaccharides, and encapsulation techniques for optimal delivery.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If a general prebiotic like FOS or GOS is used, then broad gut microbiota stimulation occurs, but selective growth of specific probiotic strains is not achieved
Solution Approach 1:
The patent applies local quality by creating a prebiotic composition with a specific oligosaccharide profile tailored to match the enzymatic capabilities of a particular probiotic strain. Instead of using general prebiotics that affect all bacteria equally, the composition is locally optimized with specific substrates that only the target strain can efficiently metabolize, thereby achieving selective growth promotion while maintaining strong growth effects.
Solution Approach 2:
The patent employs parameter changes by modifying the chemical composition parameters of the prebiotic mixture to match the specific enzymatic parameters of the target probiotic strain. By adjusting the types and ratios of oligosaccharides based on the strain's enzyme profile, the composition achieves both high selectivity and effective growth promotion for the specific strain.
2Adaptability or versatility
If existing synbiotic products are used, then probiotic and prebiotic are combined, but the prebiotic does not specifically target the probiotic species for enhanced benefits
Solution Approach 1:
The patent applies preliminary action by pre-characterizing the probiotic strain's enzymatic profile before formulating the prebiotic composition. The enzyme screening and substrate specificity analysis are performed in advance to identify the exact oligosaccharide substrates that the target strain can metabolize, ensuring that the final synbiotic product has reliable and enhanced health benefits for that specific strain.
Solution Approach 2:
The patent implements feedback by using the probiotic strain's enzymatic capabilities as the basis for designing the prebiotic composition. The strain's enzyme profile provides feedback information that directly determines the prebiotic substrate selection, creating a tailored synbiotic product where the prebiotic components specifically support the metabolism and growth of the target probiotic strain, thereby enhancing health benefit reliability.
3Quantity of substance
If non-selective prebiotics are used, then gut bacteria are stimulated, but specific probiotic strain growth is not enhanced
Solution Approach 1:
The patent applies segmentation by dividing the broad category of prebiotics into specific oligosaccharide types that correspond to the enzymatic capabilities of different probiotic strains. Instead of using a single general prebiotic, the composition is segmented into multiple specific substrates, each targeted to match the metabolic profile of the specific probiotic strain, thereby achieving both population growth and strain-specific enhancement.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition effectively promotes the growth and survival of specific probiotic strains in the gut, enhancing the gut microbiota balance and providing targeted health benefits, such as improved lactobacilli populations and increased health benefits like cholesterol reduction and diabetes modulation.
Implementation Method 1
wherein the bacterial strain is capable of producing the same growth medium by reverse enzyme reaction
Implementation Method 2
The enzyme may comprise a saccharolytic or glycosidase enzymes
Implementation Method 3
The prebiotic growth medium may comprise oligosaccharides which may be selected from β-galactosidases, α-galactosidases, α- and β-glucosidases, α-mannosidases, or β-xylosidases
Implementation Method 4
it is fermented by intestinal microflora and selectively stimulates the growth and/or activity of intestinal bacteria associated with health and well-being
Data Source
AI summary
The present invention relates to a symbiotic composition comprising a probiotic bacterial strain and a prebiotic growth medium which is specific to the growth of the probiotic bacterial strain, wherein the bacterial strain is capable of producing the same growth medium by reverse enzyme reaction. The present invention also relates to methods of producing and screening for such compositions.