Syringe-Shaped Sample Preparation With Binding-Matrix Purification
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Solution Overview
Problem
Existing sample preparation devices are inefficient and costly in purifying or isolating analytes from biological samples.
Innovation Solution
The development of pipette tip- and syringe-shaped sample preparation devices equipped with a composite frit comprising glass and polymeric material, which includes a sample binding matrix that specifically binds to analytes, allowing for methods of lysing, washing, and eluting samples using a pipette tip-shaped or syringe-shaped configuration.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional sample preparation devices are used, then analyte purification can be achieved, but the process is inefficient and costly
Solution Approach 1:
The device segments the sample preparation process into distinct functional zones within a single integrated unit: a binding matrix section for analyte capture, a washing section for contaminant removal, and an elution section for analyte release. This segmentation enables efficient purification while simplifying operation as a single disposable unit rather than multiple separate steps
Solution Approach 2:
The device employs passive flow control where the sample automatically progresses through binding, washing, and elution stages based on fluid pressure differentials and gravity, eliminating the need for external pumps or complex automation. The washing buffer and elution buffer are delivered through the same inlet, and waste collects in the syringe body cavity, creating a self-contained system that reduces operational complexity
2Productivity
If conventional sample preparation devices are used, then analyte purification can be achieved, but the process is costly
Solution Approach 1:
The device is designed as a disposable unit with a disposable syringe body and binding matrix. This eliminates the need for expensive, reusable equipment and simplifies manufacturing using inexpensive materials like plastic syringes and glass or polymer binding matrices. The low-cost disposable design reduces overall system cost while maintaining purification efficiency
Solution Approach 2:
The binding matrix uses composite materials combining glass frit with polymeric materials to create a cost-effective yet efficient analyte binding medium. This composite approach provides the necessary binding capacity and selectivity at lower material costs compared to traditional single-material systems, reducing manufacturing expenses while maintaining purification performance
3Productivity
If centrifugation is used for sample preparation, then analyte isolation can be achieved, but the process becomes complex
Solution Approach 1:
The device replaces the mechanical centrifugation system with a chromatographic binding matrix system. Instead of using centrifugal force to separate analytes, the system uses selective binding of the analyte to a functionalized matrix, allowing isolation through fluid flow control rather than mechanical rotation, thereby eliminating the need for centrifuges and simplifying the overall process
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
These devices enable high-efficiency, cost-effective isolation and purification of analytes from biological samples without the need for centrifugation, simplifying the process and reducing operational complexity.
Implementation Method 1
the analyte in the liquid lysate binds specifically to the sample binding matrix
Data Source
AI summary
A syringe-like sample preparation device is disclosed. The syringe-like sample preparation device comprises a syringe body having a proximate end, a distal end and a syringe body cavity between the proximate end and the distal end; a plunger located at the proximate end of the syringe body and movable along the syringe body cavity; and an overflow tube at the distal end of the syringe body and extending into the body cavity of the syringe body; and a sample purification tip at the distal end of the syringe body, wherein the sample purification tip comprises a sample binding matrix.