Tagatose Production Composition with Mutated Fructose-4-Epimerase
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Solution Overview
Problem
The conventional methods for producing tagatose using lactose as a raw material face instability due to fluctuating prices and supply, necessitating a stable production system using generalized saccharides like fructose, and require a high-expression system of tagatose-converting enzyme to enhance efficiency.
Innovation Solution
A mutated polynucleotide encoding Kosmotoga olearia-derived fructose-4-epimerase is introduced into Corynebacterium sp. microorganisms, increasing the expression of fructose-4-epimerase and enhancing tagatose production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional methods using lactose as raw material are employed, then tagatose production is achieved, but production stability deteriorates due to fluctuating prices and supply
Solution Approach 1:
The patent employs a universal enzyme system (fructose-4-epimerase) that can process multiple saccharide substrates including fructose, glucose, and sucrose. This multi-functional enzyme system allows the production process to adapt to different raw materials, eliminating dependency on lactose supply stability while maintaining production reliability.
Solution Approach 2:
The patent utilizes isogenic strains with modified metabolic parameters and regulated enzyme expression systems to maintain stable tagatose production. By controlling enzyme activity levels and metabolic flux through parameter optimization, the system achieves production stability independent of raw material price fluctuations.
2Productivity
If the amount of tagatose-converting enzyme is increased to enhance conversion efficiency, then productivity improves, but device complexity increases due to requirements for high-expression systems
Solution Approach 1:
The patent employs self-regulating enzyme systems where fructose-4-epimerase automatically maintains optimal activity levels through inherent metabolic feedback mechanisms. The enzyme system self-adjusts its expression and activity based on substrate availability and product accumulation, eliminating the need for complex external control systems while maintaining high productivity.
Solution Approach 2:
The patent replaces complex mechanical control systems with biological regulation mechanisms. Instead of using external devices to control enzyme expression, the system utilizes natural metabolic pathways and enzyme kinetics to regulate tagatose production, simplifying the overall system architecture while maintaining high conversion efficiency.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The mutated polynucleotide significantly increases the expression level of fructose-4-epimerase, leading to improved economic efficiency in tagatose production.
Implementation Method 1
fructose is converted into tagatose through a cellular reaction to produce tagatose
Data Source
AI summary
The present disclosure relates to a composition for tagatose production and a method for preparing tagatose using the same.