Tagged Library Chemical Entity Identification via Anti-Tag Decoding
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Solution Overview
Problem
Current methods for identifying chemical entities with preselected properties in libraries are inefficient, especially when these entities are altered, and are often confined to polypeptides, lacking versatility and requiring multi-step synthesis.
Innovation Solution
A method involving the generation of a tagged library by appending unique identifier tags to chemical entities, followed by partitioning under specific conditions, recovering anti-tags capable of interacting with the tags, and decoding these anti-tags to identify the chemical entities with desired properties, using recognition units such as nucleotides, antibodies, or proteins.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional rational approach or combinatorial library methods are used to identify chemical entities with preselected properties, then the identification process becomes complex and inefficient, but the method is confined to polypeptides and requires multi-step synthesis
Solution Approach 1:
The method segments the identification process into distinct steps: (i) generating a tagged library where each chemical entity is associated with a unique identifier tag, (ii) subjecting the library to selection conditions to partition entities with predetermined properties, (iii) recovering the tag from the partitioned entity, and (iv) decoding the tag to identify the chemical entity. This segmentation simplifies the overall complexity by breaking down the identification process into manageable, sequential operations.
Solution Approach 2:
The invention introduces a tag as an intermediary element that mediates between the chemical entity and the identification system. The tag serves as a readable marker that does not interfere with the chemical entity's properties or interactions, allowing efficient identification without requiring complex direct analysis of the entity itself. This intermediary approach resolves the contradiction by enabling simple, rapid identification while maintaining versatility across different chemical entities.
2Measurement precision
If multi-step synthesis is used to attach tags to chemical entities, then the identification accuracy improves, but the time and resources required increase significantly
Solution Approach 1:
The method employs preliminary action by pre-attaching unique identifier tags to chemical entities during library generation, before the selection and identification processes. This pre-tagging ensures that each entity is already marked for identification, eliminating the need for time-consuming tag attachment steps after synthesis. The tag is incorporated as an integral part of the library preparation, thereby maintaining identification accuracy while significantly reducing the time and resources required.
3Reliability
If the chemical entity is altered in response to the condition, then the selection specificity improves, but the identification of the original entity becomes difficult
Solution Approach 1:
The invention uses the tag as a copy or surrogate that retains the identity information of the chemical entity even when the entity itself is altered during selection. The tag remains unchanged and serves as a stable record of the entity's identity, allowing accurate identification after the entity has undergone transformations in response to selection conditions. This copying mechanism preserves entity identity information while maintaining high selection specificity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method allows for efficient identification of chemical entities with preselected properties without amplification, enabling the selection of chemical entities with specific functions from large libraries, even when they are altered, and is applicable beyond polypeptides, reducing the need for multi-step synthesis.
Implementation Method 1
an anti-tag, said anti-tag being capable of interacting with the unique identifier tag in a specific manner
Data Source
AI summary
Disclosed is a method for determining the identity of a chemical entity having a preselected property. The chemical entity is identified from a library composed of a plurality of different chemical entities each appended to unique identifier tags. An anti-tag having the capability of specifically interacting with the unique identifier tag is recovered during the method and used for identification purposes.