TBRFV-Resistant Tomato Plants Using Chromosome 11 QTL Markers
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Solution Overview
Problem
Tomato plants are susceptible to the Tomato brown rugose fruit virus (TBRFV), which causes severe fruit symptoms and is difficult to control due to mechanical transmission and lack of resistance in cultivated varieties.
Innovation Solution
Introduction of a Quantitative Trait Locus (QTL) from Solanum pimpinellifolium onto chromosome 11 of Solanum lycopersicum, flanked by specific nucleotide sequences, conferring resistance to TBRFV through molecular markers for identification and breeding.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional tomato varieties are cultivated, then they are easy to grow and manage, but they are highly susceptible to TBRFV infection causing severe fruit symptoms
Solution Approach 1:
The invention extracts and transfers a specific QTL region from Solanum pimpinellifolium (wild relative) to Solanum lycopersicum (cultivated tomato) through controlled breeding. This extracts the resistance trait from the wild species and introduces it to the cultivated variety, achieving TBRFV resistance without complex genetic modification while maintaining the ease of growing conventional tomatoes
Solution Approach 2:
The patent uses an intermediary approach by utilizing a bridging species (Solanum pimpinellifolium) that naturally possesses resistance to TBRFV. This intermediary wild relative serves as a donor of resistance genes, allowing the transfer of protective traits to cultivated tomatoes through conventional breeding methods rather than direct genetic engineering
2Reliability
If resistance genes are introduced through breeding, then resistance to viruses is improved, but the breeding process becomes more complex and time-consuming
Solution Approach 1:
The invention performs preliminary action by pre-identifying and characterizing the specific QTL region on chromosome 11 from Solanum pimpinellifolium that confers TBRFV resistance. Molecular markers are developed in advance to track this QTL during breeding, allowing breeders to efficiently select resistant plants without lengthy trial-and-error breeding processes
Solution Approach 2:
The patent replaces traditional mechanical phenotypic selection (observing disease symptoms) with molecular marker-based selection. By using DNA markers linked to the resistance QTL, breeders can identify resistant plants at the molecular level early in the breeding process, dramatically reducing the time required compared to waiting for disease manifestation and phenotypic evaluation
3Object-affected harmful factors
If QTL from wild species is introduced, then resistance to TBRFV is achieved, but the genetic uniformity of cultivated varieties is reduced
Solution Approach 1:
The invention applies local quality by introducing only the specific QTL region from Solanum pimpinellifolium that confers TBRFV resistance, rather than introducing entire genomes or multiple unrelated traits. This targeted approach introduces minimal foreign genetic material (a localized chromosomal segment) while achieving the desired resistance, thereby maintaining overall genetic uniformity and consistency of the cultivated tomato variety
Data Source
AI summary
The invention relates to a Solanum lycopersicum plant that is resistant to TBRFV, which plant comprises a QTL on chromosome 11 between SEQ ID NO: 1 and 53. The presence of the QTL on chromosome 11 is identified by use of at least one of the markers selected from the group comprising SEQ ID NOS: 4 to 52. The QTL is as comprised in the genome of a Solanum lycopersicum plant representative seed of which was deposited with the NCIMB under deposit number NCIMB 42882, NCIMB 42885, NCIMB 42887, or NCIMB 42890.