Optical Imaging System Using Time Delay Integration Line Scan Camera
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Solution Overview
Problem
Existing optical imaging systems for biochemical substance analysis face challenges such as slow acceleration and deceleration of the platform, limited field of view and numerical aperture of the microscope objective, and high-throughput bottlenecks in sequencing processes.
Innovation Solution
The optical imaging system employs a time delay integration line scan camera, a specialized objective lens with a large field of view and numerical aperture, and a focusing system that adjusts the distance between the objective lens and the sample carrier in real time to maintain image quality and compensate for environmental changes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Area of stationary object
If sequential scanning of multiple rows or columns is used to photograph the entire sequencing chip, then the entire chip area can be detected, but the photographing time increases and detection throughput decreases
Solution Approach 1:
The patent transitions from sequential row-by-row scanning to simultaneous parallel imaging by capturing multiple rows or columns at once. This dimensional change in the imaging approach allows the entire chip area to be photographed in a single exposure, eliminating the time penalty of sequential scanning while maintaining complete area coverage.
2Speed
If the platform accelerates and decelerates quickly to stop for photographing, then high-speed operation is maintained, but the acceleration and deceleration time increases
Solution Approach 1:
The patent enables continuous high-speed operation by eliminating the need for repeated acceleration and deceleration cycles. By capturing multiple rows or columns simultaneously, the platform can maintain constant velocity throughout the scanning process, with acceleration and deceleration occurring only at the beginning and end of the entire sequencing chip scan, thereby maintaining speed while reducing total acceleration/deceleration time.
3Device complexity
If a conventional microscope objective is used, then the system is simple, but it cannot achieve both large field of view and large numerical aperture
Solution Approach 1:
The patent divides the imaging task into multiple simultaneous captures of different rows or columns. This segmentation allows each individual image to be captured with a conventional objective lens at standard speed, while the overall system achieves high throughput through parallel processing of multiple segmented regions in a single exposure cycle.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This configuration reduces the photographing time of each sample, improves detection flux, enhances imaging quality by preventing interference between excitation and detection light, and ensures high-throughput optical sequencing with improved uniformity and energy utilization of the illumination spot.
Implementation Method 1
The lighting module is configured for outputting excitation light, the excitation light is configured to excite the sample to generate excited light
Data Source
AI summary
An optical imaging system (1) is configured for photographing a sample and includes a lighting module (11) and an imaging module (15). The lighting module (11) is configured for outputting excitation light, the excitation light is configured to excite the sample to generate excited light, the imaging module (15) comprises a time delay integration line scan camera (151), the time delay integration line scan camera (151) is configured to record the excited light. A biochemical substance detection system using the optical imaging system (1) is also provided, improving the detection flux.