Templated Nanoconjugates for Nucleic Acid Delivery
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Delivering nucleic acids into mammalian cells is challenging due to cellular resistance and degradation, and existing nanomaterials face concerns over core material toxicity and clearance in vivo.
Innovation Solution
Development of templated nanoconjugates with crosslinked biomolecules that can be activated to form hollow structures, allowing for non-toxic penetration of cellular membranes and stable interaction with biological systems without external transfection agents.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If nucleic acids are delivered into mammalian cells, then gene expression regulation is achieved, but cellular resistance and degradation mechanisms prevent successful delivery
Solution Approach 1:
The patent uses inorganic nanoparticle templates as intermediary carriers to mediate the delivery of nucleic acids into mammalian cells. These templates provide a protective interface that shields nucleic acids from cellular degradation mechanisms while facilitating entry through cellular membranes, thereby resolving the contradiction between delivery efficiency and cellular resistance
Solution Approach 2:
The invention creates composite nanoconjugates combining inorganic nanoparticle templates with crosslinked biomolecule shells. This composite structure integrates the beneficial properties of both components: the inorganic core provides structural stability and protective functions, while the organic shell enables biological compatibility and targeted delivery, overcoming the limitations of pure nucleic acid delivery
2Reliability
If polyvalent inorganic nanomaterials are used as therapeutic agents, then high cellular uptake and stability are achieved, but core material toxicity and clearance issues arise
Solution Approach 1:
The patent fundamentally changes the physical state and composition parameters of the nanoparticle core by using water-soluble inorganic materials that can be completely removed after template-directed assembly. This parameter change allows achieving high stability and cellular uptake during the delivery process while eliminating toxicity and clearance issues through complete removal of the inorganic core after delivering the therapeutic payload
Solution Approach 2:
The inorganic nanoparticle template performs preliminary structural organization and stabilization of the biomolecule shell before the actual therapeutic function is executed. The template directs the self-assembly and crosslinking of biomolecules into the desired nanoconjugate structure, then is removed to leave a stable, functional, and non-toxic organic nanoconjugate that maintains the benefits of the inorganic template without its harmful effects
3Reliability
If external transfection agents are used to deliver nucleic acids, then delivery efficiency improves, but toxicity increases
Solution Approach 1:
The patent employs self-service mechanisms where the templated nanoconjugates inherently possess the ability to penetrate cellular membranes and deliver nucleic acids without requiring external transfection agents. The nanoconjugate structure itself provides the necessary functions for efficient delivery through its engineered surface properties and cellular uptake mechanisms, eliminating the need for toxic external agents while maintaining high delivery efficiency
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The templated nanoconjugates achieve high cellular uptake, resistance to nuclease degradation, and stability in biological media, reducing toxicity and enabling effective therapeutic and diagnostic applications.
Implementation Method 1
biomolecules bearing one or more moieties that can be crosslinked are activated, which initiates crosslinking reactions between biomolecules
Implementation Method 2
biomolecules bearing one or more moieties that can be crosslinked are activated, which initiates crosslinking reactions between biomolecules
Data Source
AI summary
The present disclosure is directed to compositions comprising templated nanoconjugates and methods of their use.