Immunochromatography Test Kit Backflow Prevention Region
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Solution Overview
Problem
Test kits using immunochromatography face challenges in achieving rapid and highly sensitive detection due to retention of labeling substances caused by disturbances in the flow of liquid samples, leading to excessive time for reliable development beyond the test line.
Innovation Solution
The test kit design includes a multifunctional member with distinct regions for sample dropping, labeling substance holding, and backflow prevention, where the labeling substance holding region is connected to the immobilizing membrane with a non-containing portion upstream, and a backflow prevention region with higher absorbency than the dropping region, facilitating smooth flow and preventing backflow.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If the labeling substance holding pad is used with uniform labeling substance distribution, then the structure is simple, but the labeling substance is retained due to flow disturbances
Solution Approach 1:
The labeling substance holding pad is segmented into a labeling substance holding region and a non-containing portion. This segmentation allows the liquid sample to flow smoothly from the non-containing portion to the holding region, preventing flow disturbances that cause labeling substance retention, while maintaining structural simplicity
Solution Approach 2:
The pad structure implements local quality by having different regions with different functions: the non-containing portion facilitates smooth flow, while the holding region contains the labeling substance. This local differentiation resolves the contradiction between structural simplicity and detection reliability
2Speed
If the liquid sample flows in with high intensity, then the development speed is fast, but flow disturbances occur causing labeling substance retention
Solution Approach 1:
The non-containing portion is positioned upstream of the labeling substance holding region to preliminarily guide and smooth the liquid sample flow before it enters the holding region. This preliminary action prevents flow disturbances that would cause labeling substance retention while maintaining fast development speed
3Loss of time
If the test kit enables rapid detection, then the detection time is short, but the labeling substance may not be reliably developed beyond the test line
Solution Approach 1:
By segmenting the pad into regions with different absorbencies (dropping region with lower absorbency, backflow prevention region with higher absorbency), the system achieves rapid liquid sample flow while preventing backflow and ensuring complete development of the labeling substance beyond the test line, resolving the time-reliability contradiction
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This configuration enables rapid and highly sensitive detection by ensuring the labeling substance reaches the detection zone efficiently, reducing retention and promoting accurate identification of the test line.
Implementation Method 1
allowing a liquid sample to develop downstream by capillary phenomenon
Data Source
AI summary
The present invention provides a test kit capable of rapid and highly sensitive detection.The test kit is provided with a first member, which contains a region in which is held a labeling substance with a label immobilized on a substance that specifically binds with a substance to be detected, and a second member, which has a detection zone where the labeling substance is captured through the substance to be detected, which is connected downstream of the first member in the developing direction, and which allows the labeling substance contained in a liquid sample that flows in from the first member, to develop into the detection zone. The first member has a dropping region located furthermost upstream and containing a portion onto which the liquid sample is dropped, a labeling substance holding region connected to the second member and having a containing portion that contains the labeling substance and a non-containing portion that is located upstream of the containing portion and that does not contain the labeling substance, and a backflow prevention region, which is connected between the dropping region and the non-containing portion of the labeling substance holding region, and in which absorbency is set higher than in the dropping region.