Immunochromatographic Test Strip Backing for Bleaching Control
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Solution Overview
Problem
Immunochromatographic test strips experience a bleaching phenomenon and variations in detection values due to non-specific light reflections, affecting measurement accuracy and reproducibility, particularly at low analyte concentrations.
Innovation Solution
Incorporating an opaque base material on the back surface of the porous membrane in the test strip, preferably white and with a thickness of 30 μm to 200 μm, to reduce non-specific light reflections and stabilize detection values.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a transparent base material is used on the back surface of the porous membrane, then light transmission is maintained, but the bleaching phenomenon occurs causing measurement waveform disturbance and reduced detection accuracy
Solution Approach 1:
An opaque base material is introduced as an intermediary layer between the porous membrane and the external environment. This intermediary prevents the harmful bleaching effect while maintaining the functional integrity of the immunochromatographic detection system.
Solution Approach 2:
The base material is changed from transparent to opaque (typically white), fundamentally altering its optical properties. This color/transparency change prevents light transmission that causes bleaching, thereby eliminating the harmful effect while preserving detection capabilities.
2Ease of manufacture
If an opaque base material is placed on the back surface of the porous membrane, then the bleaching phenomenon is prevented and visibility is enhanced, but the structural complexity of the test strip increases
Solution Approach 1:
The opaque base material serves multiple functions simultaneously: it prevents bleaching, enhances visual detection of the test line, and provides structural support. By consolidating these functions into a single component, the overall device complexity is minimized despite the added functionality.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The opaque base material prevents bleaching and enhances visibility during visual detection, reducing variations in measurement values and improving reproducibility.
Implementation Method 1
an opaque base material is placed on a back surface of the porous membrane
Implementation Method 2
the analyte specifically binds to the labeled antibody to form a complex
Implementation Method 3
The complex is developed in the development region downstream direction
Data Source
AI summary
A problem to be solved is to provide a test strip capable of avoiding occurrence of a so-called bleaching phenomenon in an immunochromatographic test strip to enable better detection and visibility. Another problem to be solved is to provide an immunochromatographic test strip capable of preventing a variation in detection value on a test line.The problems are solved by an immunochromatographic test strip including at least a porous membrane and having a sample application region, a development region, and a detection region in which a specific binding substance is immobilized, wherein the detection region is immobilized on a front surface of the porous membrane, and wherein an opaque base material having a thickness of 30 μm or more and 200 μm or less is placed on the back surface of the porous membrane.