Test Substance Detection via Signal Pattern Comparison
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Solution Overview
Problem
Conventional detection methods for test substances face challenges with complicated B/F separation and nonspecific adsorption, leading to difficulties in specific detection.
Innovation Solution
A method involving a capturing body 1 fixed to a substrate and a capturing body 2 with a label, brought into contact in a liquid, followed by repeated dissociation and contact steps to compare signal patterns, allowing for specific detection of the test substance by identifying signals at substantially the same position.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional detection methods (ELISA) are used to detect test substance, then detection capability is achieved, but complicated B/F separation work is required and nonspecific adsorption occurs
Solution Approach 1:
The patent extracts and removes the B/F separation step from the detection process by using magnetic particles that can be easily separated from the solution through magnetic field application, eliminating the need for complex filtration or centrifugation procedures while maintaining detection capability
Solution Approach 2:
The patent combines the detection function with easy separation functionality by incorporating magnetic particles into the detection system, allowing the detection complex to be both detected and easily separated through magnetic field application, thus resolving the complexity issue
2Reliability
If conventional detection methods are used to detect test substance, then detection capability is achieved, but nonspecific adsorption remains as background signal
Solution Approach 1:
The patent applies local quality by using specific magnetic particles with targeted binding properties that selectively bind to the test substance, ensuring that only relevant signals are detected while nonspecific adsorption is minimized through localized specific binding interactions
Solution Approach 2:
The patent employs a feedback mechanism where magnetic particles are used to concentrate and isolate the test substance, allowing for repeated washing and separation steps that progressively remove nonspecific background signals while retaining the specific test substance signal, thereby improving measurement precision
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach effectively reduces nonspecific adsorption and enhances the specificity of test substance detection by comparing label-derived signal patterns before and after dissociation, improving the accuracy of signal indication.
Implementation Method 1
a capturing body 1 that has binding properties to the test substance and is fixed to a substrate, and a capturing body 2 that has binding properties to the test substance and contains a label
Data Source
AI summary
Disclosed is a method for detecting a test substance, including a first contact step of bringing the test substance, a capturing body 1 that has binding properties to the test substance and is fixed to a substrate, and a capturing body 2 that has binding properties to the test substance and contains a label into contact with each other in a liquid to arrange at least a part of the capturing body 2 on the substrate; after the first contact step, a first dissociation step of dissociating a part of substances containing the capturing body 2 from the substrate and releasing it into the liquid; after the first dissociation step, a second contact step of bringing the test substance, the capturing body 1 that has binding properties to the test substance and is fixed to a substrate, and the capturing body 2 that has binding properties to the test substance and contains a label into contact with each other in the liquid to arrange at least a part of the capturing body 2 on the substrate; after the second contact step, a second dissociation step of dissociating a part of substances containing the capturing body 2 from the substrate and releasing it into the liquid; and a detection step of comparing a label-derived signal arrangement pattern on the substrate after dissociation in the first dissociation step with a label-derived signal arrangement pattern on the substrate after dissociation in the second dissociation step and detecting signals at substantially the same position as a signal indicating the test substance.