Tetraploid Rice Breeding Method for Seed Setting Rate
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Solution Overview
Problem
The bottleneck of low seed setting rate in polyploid rice breeding limits the production of polyploid rice, hindering the increase in food production needed to address global food shortages.
Innovation Solution
A breeding method that converts diploid photo-thermo-sensitive genetic male sterile lines to tetraploid rice lines with polyploid meiosis stability (PMeS) through hybridization, doubling culture, back-crossing, and self-crossing, resulting in stable and consistent tetraploid photo-thermo-sensitive genetic male sterile lines with high seed setting rates.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If polyploid rice breeding is conducted to increase food production, then the potential yield and heterosis are improved, but the seed setting rate is reduced
Solution Approach 1:
The patent changes the ploidy level parameter from diploid to tetraploid, creating polyploid rice lines with modified chromosomal sets. This parameter change enables the rice to exhibit both high seed setting rates and strong heterosis, resolving the contradiction between productivity improvement and seed setting rate maintenance
Solution Approach 2:
The patent develops photo-thermo-sensitive genetic male sterile lines that dynamically adjust their fertility based on environmental conditions (light and temperature). The lines exhibit male sterility under long-day high-temperature conditions for hybrid production, but regain fertility under short-day low-temperature conditions for seed recovery, enabling flexible control over seed setting rates throughout the breeding process
2Device complexity
If diploid rice lines are used for hybridization, then the breeding process is simple, but the heterosis and yield potential are limited
Solution Approach 1:
The patent systematically changes the ploidy parameter from diploid (2n) to tetraploid (4n) through controlled hybridization and chromosome doubling. This parameter escalation unlocks superior heterosis and yield potential while the developed breeding system maintains operational simplicity through standardized protocols for polyploid rice reproduction and hybridization
Data Source
AI summary
A breeding method of a polyploid rice photo-thermo-sensitive genetic male sterile line includes determining a diploid rice line with photo-thermo-sensitive genetic male sterility or PMeS characteristic as a parent; carrying out hybridization on a diploid photo-thermo-sensitive genetic male sterile line and a diploid PMeS gene line, carrying out doubling culture on a young ear of a hybrid plant into a hybrid tetraploid; back-crossing the hybrid tetraploid with a tetraploid photo-thermo-sensitive genetic male sterile line; selecting a tetraploid male sterile plants from the back-crossed progeny, self-crossing during a low-temperature and short-day fertile period, and then carrying out composite hybridization with another tetraploid rice line having PMeS gene; selecting tetraploid male sterile plants, and detecting the stability of tetraploid male sterile plants after multiple generations of continuous self-crossing; and determining the stable and consistent tetraploid rice sterile line as the polyploid rice photo-thermo-sensitive genetic male sterile line, named as PSXXX.