Thomsen-Friedenreich Antigen Antibody for MSI Detection
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Solution Overview
Problem
Current methods for diagnosing Microsatellite Instability (MSI) in gastric and colorectal cancers are resource-intensive and time-consuming, often requiring multiple markers and complex procedures, which hinders timely and efficient identification and stratification of patients for treatment.
Innovation Solution
The use of antibodies with affinity for the Thomsen-Friedenreich (TF) antigen, such as 3C9, SPM320, A68/B-A11, and Jacalin, in a composition for in vitro diagnostics, allowing for a single marker-based, rapid, sensitive, and specific assay to detect MSI in biological samples through immunohistochemistry or ELISA, facilitating MSI detection in gastric and colorectal cancers.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If multiple markers and complex procedures are used for diagnosing MSI, then measurement precision is improved, but device complexity and loss of time increase
Solution Approach 1:
The invention extracts and isolates a single critical marker (Thomsen-Friedenreich antigen) from the complex panel of multiple markers traditionally used for MSI diagnosis. By focusing on this one specific antigen that is highly associated with MSI status, the method simplifies the diagnostic procedure while maintaining high measurement precision through targeted detection.
Solution Approach 2:
The Thomsen-Friedenreich antigen serves as a universal marker that can indicate MSI status across different cancer types (gastric and colorectal cancers). This single marker performs the diagnostic function that previously required multiple different markers, reducing procedural complexity while maintaining diagnostic accuracy.
2Measurement precision
If multiple markers and complex procedures are used for diagnosing MSI, then measurement precision is improved, but loss of time increases
Solution Approach 1:
The invention extracts and isolates a single critical marker (Thomsen-Friedenreich antigen) from the complex panel of multiple markers traditionally used for MSI diagnosis. By focusing on this one specific antigen that is highly associated with MSI status, the method simplifies the diagnostic procedure while maintaining high measurement precision through targeted detection.
Solution Approach 2:
The invention skips the time-consuming steps of analyzing multiple markers by directly targeting the Thomsen-Friedenreich antigen. This allows the diagnostic process to rush through to a conclusion more quickly by focusing resources on the single most predictive marker rather than sequentially evaluating multiple markers.
3Productivity
If a single marker-based assay is used for detecting MSI, then productivity is improved, but measurement precision may worsen
Solution Approach 1:
The invention changes the parameter being measured by selecting the Thomsen-Friedenreich antigen expression level as the single critical parameter for MSI detection. This specific antigen has been shown to have high sensitivity and specificity for MSI status, allowing a single-parameter assay to achieve precision comparable to or exceeding multi-marker methods while dramatically improving productivity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach provides a highly specific and sensitive method for identifying MSI status, with significant association between TF antigen expression and MSI, offering improved prognostic value and potential for predicting response to immunotherapy, while being more efficient and cost-effective than existing methods.
Implementation Method 1
The present invention refers to the use of an antibody with affinity for the Thomsen-Friedenreich (TF) antigen or a fragment thereof
Data Source
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Figure 2
AI summary
The present invention refers to the use of antibodies against the Thomsen-Friedenreich (TF) as reagents for in vitro diagnostics of Microsatellite Instability (MSI). Another aspect of this invention refers to a method for the in vitro diagnostics of MSI comprising the said composition employing the anti-TF antigen antibodies and measuring the expression of the TF antigen in a tissue sample. The present invention further refers to a kit comprising the above mentioned compositions for detecting MSI by conducting the present invention's method. The use, composition, methods and kit may be advantageously applied to significantly facilitate the identification of MSI in the clinical setting through a single marker-based sensitive and specific detection of MSI in patients with cancers of gastric, colorectal and other tissues for diagnostics, prognostics or prediction of response to treatment.