Thermophilic Microbial Fermentation for Soil Nitrate Reduction

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Solution Overview

Problem

Conventional agriculture relies heavily on chemical fertilizers, leading to soil and water pollution and greenhouse gas emissions, as well as high-temperature disorders and disease outbreaks in crops, necessitating an environmentally friendly and multifunctional solution.

Innovation Solution

A microbial material produced through fermentation using a mixture of thermophilic microorganisms, including Bacillus brevis and Bacillus stearothermophilus, which reduces nitrate ions in soil, promotes denitrification, and enhances plant functionality by activating nitrate transporters, increasing antioxidant production, and providing heat resistance.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If chemical fertilizers are used to improve crop production, then productivity increases, but soil and water pollution occurs and greenhouse gas emissions increase

Engineering Contradiction:
Improvecrop productionVSAvoidnitrate pollution and greenhouse gas emissions
Core Design Contradiction:
ProductivityVSObject-generated harmful factors

Solution Approach 1:

The patent converts harmful nitrate ions in soil into beneficial nitrogen gas through denitrification. Thermophilic microorganisms are introduced to transform excess nitrate (a pollutant) into nitrogen gas (a harmless substance), thereby eliminating pollution while maintaining soil fertility for crop production.

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

Thermophilic microorganisms serve as intermediaries between chemical fertilizers and crops. Instead of directly applying chemical fertilizers that cause pollution, the microorganisms process the nutrients and deliver them to plants in a controlled manner, reducing harmful emissions while maintaining productivity.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If conventional agricultural methods are used to maintain crop health, then productivity is maintained, but high-temperature disorders and disease outbreaks occur

Engineering Contradiction:
Improvecrop yieldVSAvoidhigh-temperature disorder and disease outbreaks
Core Design Contradiction:
ProductivityVSObject-affected harmful factors

Solution Approach 1:

The patent enhances plants' self-protection capabilities by introducing thermophilic microorganisms that stimulate the plants' own defense mechanisms. The microorganisms trigger heat shock protein production and antioxidant synthesis within the plants, enabling them to self-protect against high-temperature stress and diseases without external intervention.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The thermophilic microorganisms are applied in advance to prepare plants for environmental stresses. By pre-stimulating heat shock protein expression and antioxidant systems before actual stress occurs, the plants are primed to resist high-temperature disorders and disease outbreaks, maintaining productivity under adverse conditions.

Inventive Principle:
Principle #10Preliminary action

3Object-generated harmful factors

If thermophilic microorganisms are used for denitrification and plant enhancement, then environmental sustainability improves, but manufacturing complexity increases

Engineering Contradiction:
Improvenitrate pollution and greenhouse gas emissionsVSAvoidfermentation process complexity
Core Design Contradiction:
Object-generated harmful factorsVSDevice complexity

Solution Approach 1:

The patent combines multiple functions into a single microbial preparation: denitrification, plant growth promotion, stress resistance enhancement, and pathogen suppression are all achieved through one fermented microbial product. This consolidation simplifies application compared to using multiple separate treatments.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The fermentation process parameters (temperature, time, microbial strains) are optimized to produce a stable, standardized product. By controlling these parameters, the complex biological process yields a consistent preparation that is easy to store and apply, reducing manufacturing complexity despite the sophisticated biochemistry involved.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS11118158B2Microbial material for reducing soil/water quality contamination, restricting warming gas generation, and improving plant function, and method for manufacturing fermentation product
Publication Date: 2021.09.14 JAPAN ECO SCIENCE CO LTD
  • US11118158B2 patent drawing
  • US11118158B2 patent drawing
  • US11118158B2 patent drawing

AI summary

Provided are a functional microbial material and a method for producing a fermentation product, wherein the functional microbial material and the fermentation product are environment-friendly and improve the quality of crops. The present invention relates to a microbial material and a method for producing a fermentation product wherein the microbial material and the fermentation product are obtained by fermentation of a fermentation raw material including a plant-derived raw material and an animal-derived raw material using a population of microorganisms including a plurality of species of thermophilic microorganisms, and lead to a remedy for soil and water pollution and to the inhibition of greenhouse gas generation, as well as contribute to improvements in the functionality of plants, particularly the expression of genes involved in biophylaxis and resistant to high-temperature disorder, and an increase in antioxidant components. Quality enhancement of plants is promoted without genetic recombination, using a population of microorganisms that have a function to restore the environment and a function as an elicitor for the plant. Further, burdens on the natural environment are maintained and restored through normal production activities.