Thermostable Glucanase Polypeptide for High-Temperature Feed Processing
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Solution Overview
Problem
Existing enzymes lack thermal tolerance to withstand high-temperature processes necessary for feed applications, hindering efficient degradation of anti-nutritional glucan in animal feed.
Innovation Solution
A polypeptide with SEQ ID NO: 4 having glucanase activity, along with a polynucleotide encoding it, is provided, which exhibits improved thermal tolerance and stability, enabling effective glucan degradation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If existing enzymes are used for glucan degradation in feed, then glucanase activity is provided, but thermal tolerance is insufficient to withstand high-temperature processes
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of the glucanase enzyme to achieve optimal thermal stability. Specifically, the enzyme was engineered with specific amino acid substitutions that enhance its structural stability at high temperatures while preserving its catalytic activity for glucan degradation in feed applications
2Reliability
If thermal tolerance is improved through enzyme engineering, then high-temperature process resistance is achieved, but enzyme complexity increases
Solution Approach 1:
The patent applies local quality by introducing specific amino acid modifications at particular positions within the enzyme structure rather than进行全面改造. This targeted approach enhances thermal stability through localized structural changes while maintaining the overall simplicity and functionality of the enzyme for feed applications
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The polypeptide maintains significant activity in a wide temperature range, enhancing the efficiency of glucan degradation in various industrial fields, including feed, baking, and pulp bleaching.
Implementation Method 1
a polypeptide of SEQ ID NO: 4 having glucanase activity
Data Source
AI summary
The present disclosure relates to the polypeptide comprising SEQ ID NO: 4.