Thrips-Resistant Brassica oleracea via Monogenic Determinant
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Solution Overview
Problem
Current Brassica oleracea varieties lack high resistance to Thrips tabaci, leading to significant yield loss and environmental concerns with ineffective insecticide use due to the pest's protection within cabbage heads and difficulty in detection.
Innovation Solution
Development of Brassica oleracea plants with a genetic determinant conferring high resistance to Thrips tabaci, inherited as a monogenic additive trait, allowing for higher and more easily incorporated resistance levels compared to polygenic traits, with the genetic determinant located on chromosome 2 and linked to specific markers.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Object-affected harmful factors
If insecticides are applied to control thrips in cabbage, then thrips population is reduced, but environmental pollution increases and effectiveness is limited due to thrips protection within closed leaves
Solution Approach 1:
The patent changes the biological parameter of the cabbage plant by introducing a genetic determinant that confers resistance to thrips. This genetic modification alters the plant's susceptibility parameter, enabling it to resist thrips feeding and reproduction without requiring chemical interventions, thereby eliminating environmental pollution while effectively controlling the harmful factor.
2Object-affected harmful factors
If insecticides are applied to cabbage leaves, then some thrips are killed, but resistance is limited to outer leaves (up to sixth exterior leaf) while thrips infestation occurs from second to fifteenth exterior leaf
Solution Approach 1:
The patent modifies the plant's biological parameter by introducing a genetic determinant that provides system-wide resistance throughout the entire cabbage plant, including all leaves from the second to fifteenth exterior leaf. This genetic solution eliminates the coverage limitation of insecticides, which only affect outer leaves, by making the entire plant resistant to thrips infestation.
3Ease of manufacture
If conventional cabbage varieties are grown, then production is simple, but yield loss occurs due to thrips infestation affecting 75% of total acreage
Solution Approach 1:
The patent changes the genetic parameter of cabbage varieties by introducing a specific genetic determinant that confers thrips resistance. This genetic modification enables the development of new cabbage varieties that maintain ease of cultivation while achieving high resistance to thrips, thereby preventing the 75% yield loss that affects conventional varieties and improving overall productivity.
4Productivity
If thrips-resistant Brassica oleracea varieties are developed, then yield loss is reduced, but no highly resistant varieties are currently known as resistance is typically polygenic and complex to incorporate
Solution Approach 1:
The patent fundamentally changes the genetic architecture of thrips resistance by introducing a single monogenic additive determinant instead of the typical polygenic complex. This simplification allows for easy incorporation of high-level resistance into new Brassica oleracea varieties through conventional breeding, eliminating the complexity associated with managing multiple resistance genes while achieving superior productivity and yield protection.
Data Source
AI summary
The invention relates to a Brassica plant in particular a Brassica oleracea plant comprising a genetic determinant, which when homozygously present confers high resistance against Thrips tabaci, and which is as found in plants grown from seeds of which a representative sample is deposited with the NCIMB under NCIMB accession number 41760. Preferably, the plant is homozygous for the genetic determinant and resistant against Thrips tabaci. The genetic determinant in the seeds of NCIMB deposit 41760 is located on chromosome 2 and linked to marker BO00200 (SEQ ID NO: 1) and/or marker BO00277 (SEQ ID NO: 2) and/or BO00602 (SEQ ID NO: 5).