Thrombocyte Stabilization Formulations at Ambient Temperatures
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Solution Overview
Problem
Existing methods fail to maintain the stability and functionality of thrombocytes at ambient temperatures for extended periods, making accurate analysis and storage challenging for research, diagnostic, and therapeutic purposes.
Innovation Solution
Formulations comprising a pH buffer, an anticoagulant, a non-reducing sugar, a polyol, and a functionalized carbohydrate are used to stabilize thrombocytes in an inactivated state at ambient temperatures for at least 24 hours, ensuring their functionality and ability to be activated post-collection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If anti-coagulants are added to maintain thrombocytes in an inactivated state at ambient temperatures, then thrombocyte aggregation is prevented, but thrombocyte stability and functionality are lost after 24 hours
Solution Approach 1:
The invention changes the chemical parameters of the storage medium by incorporating specific concentrations of glucose (5.5-27.7 mM), fructose (5.5-27.7 mM), and EDTA (0.02-0.2 mM) to create optimal conditions for thrombocyte stabilization. This parameter optimization allows thrombocytes to remain stable and functional at ambient temperatures for extended periods up to 7 days
Solution Approach 2:
The invention uses a composite formulation combining multiple substances (glucose, fructose, EDTA, and other components) working together to achieve thrombocyte stabilization. This composite approach addresses the limitations of single anti-coagulants by providing both aggregation prevention and long-term stability through synergistic interactions among the components
2Ease of operation
If thrombocytes are stored at ambient temperatures, then storage and shipping logistics are simplified, but thrombocyte activation and aggregation occur rapidly
Solution Approach 1:
The invention introduces EDTA as an intermediary substance that mediates between the thrombocytes and the ambient environment. EDTA chelates calcium ions, preventing calcium-dependent activation pathways while allowing the thrombocytes to remain in an inactivated state during ambient temperature storage and shipping
3Duration of action of stationary object
If thrombocytes are maintained in an inactivated state for extended periods, then storage time is increased, but post-collection activation capability is reduced
Solution Approach 1:
The invention provides beforehand cushioning by incorporating protective agents (glucose, fructose, EDTA) in the storage formulation that prevent irreversible damage to thrombocytes during extended storage. These agents cushion against metabolic stress and maintain membrane integrity, ensuring that thrombocytes retain their activation capability even after 7 days of ambient temperature storage
Data Source
AI summary
Provided herein are formulations and methods for the stabilization of one or more thrombocytes at ambient temperatures. Also provided are formulations and methods for the stabilization of one or more thrombocytes in an inactivated state in a blood sample at ambient temperatures. Further provided are articles of manufacture and kits and methods for substantially stable storage of one or more thrombocyte at ambient temperatures.