Thyroid Hormone-Immobilized Carrier pH Stabilization
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Solution Overview
Problem
The existing three-reagent kit for thyroid hormone measurement is cumbersome, time-consuming, and costly due to the need to mix three reagents every time, and the thyroid hormone-immobilized carrier has low stability when stored, reducing its activity over time.
Innovation Solution
A two-reagent kit is proposed, where a thyroid hormone-immobilized carrier is stabilized by setting the pH of the solvent in a range of 8.7 to 11.5, allowing for long-term storage and simplifying the measurement process by eliminating the need for frequent reagent preparation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If a two-reagent kit using thyroid hormone-immobilized carrier is used, then the measurement process is simplified and cost is reduced, but the stability of the thyroid hormone-immobilized carrier deteriorates during storage
Solution Approach 1:
The patent applies parameter changes by optimizing the pH of the solvent to a specific range (8.7-11.5) to stabilize the thyroid hormone-immobilized carrier. This chemical parameter adjustment prevents degradation during storage while maintaining the simplified two-reagent kit structure, resolving the contradiction between process simplification and carrier stability.
2Loss of time
If the thyroid hormone-immobilized carrier is stored for long periods, then the measurement convenience is improved, but the activity of the carrier is reduced
Solution Approach 1:
The patent maintains carrier activity during long-term storage by changing the solvent pH parameter to 8.7-11.5. This optimized pH range prevents activity loss over time, enabling the carrier to be stored for extended periods without degradation, thus resolving the contradiction between storage convenience and activity maintenance.
3Stability of the object's composition
If the three-reagent kit is used, then the carrier stability is maintained, but the operation becomes complicated and time-consuming
Solution Approach 1:
The patent merges the thyroid hormone with the immobilized carrier to create a pre-prepared two-reagent system. This combination eliminates the need for separate preparation steps required in three-reagent kits, simplifying operation while the optimized pH condition maintains stability, thus resolving the contradiction between ease of operation and stability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The stabilization of the thyroid hormone-immobilized carrier at a specific pH range maintains its activity for an extended period, enabling easy and cost-effective thyroid hormone measurement in clinical examinations and other applications.
Implementation Method 1
a pH of the solvent containing the thyroid hormone-immobilized carrier is in a range from 8.7 to 11.5
Data Source
AI summary
A liquid reagent of a stabilized thyroid hormone-immobilized carrier, by which a thyroid hormone can be measured easily in a short time and at low cost, is provided. The liquid reagent of a thyroid hormone-immobilized carrier according to the present invention includes: a thyroid hormone-immobilized carrier; and a solvent, and a pH of the solvent containing the thyroid hormone-immobilized carrier is in a range from 8.7 to 11.5. The detection of a thyroid hormone using the liquid reagent of the present invention can be carried out by competitively binding a thyroid hormone in a sample and the thyroid hormone-immobilized carrier in the liquid reagent with an anti-thyroid hormone antibody and detecting a composite of the thyroid hormone-immobilized carrier and the anti-thyroid hormone antibody.