TIC5290 Insecticidal Protein Overcoming Pest Resistance
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Solution Overview
Problem
Current insecticidal toxin proteins used in transgenic crops are facing resistance issues from target pests, necessitating the development of new proteins with broader spectrum efficacy and different modes of action to maintain effective pest control.
Innovation Solution
A novel class of pesticidal proteins, TIC5290, and related variants are disclosed, which exhibit insecticidal activity against Coleopteran, Lepidopteran, and Hemipteran pests, including Western Corn Rootworm, by encoding sequences that can be expressed in plants or produced through recombinant methods, offering alternative modes of action.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If current insecticidal toxin proteins are used in transgenic crops, then pest control is achieved, but resistance development by target pests occurs
Solution Approach 1:
The patent divides the existing insecticidal protein market into multiple segments by introducing novel protein classes (Cry15, Cry51, Cry52, Cry53, Cry54, Cry55, Cry56, Cry57, Cry58, Cry59, Cry60, Cry61, Cry62, Cry63, Cry64, Cry65, Cry66, Cry67, Cry68, Cry69, Cry70, Cry71, Cry72, Cry73, Cry74, Cry75, Cry76, Cry77, Cry78, Cry79, Cry80, Cry81, Cry82, Cry83, Cry84, Cry85, Cry86, Cry87, Cry88, Cry89, Cry90, Cry91, Cry92, Cry93, Cry94, Cry95, Cry96, Cry97, Cry98, Cry99, Cry100) with different modes of action. Each protein class targets specific pest groups through unique mechanisms, preventing any single resistance mechanism from becoming universally effective.
Solution Approach 2:
The patent changes the fundamental parameters of insecticidal proteins by discovering and utilizing entirely new protein families with distinct structural and functional characteristics. These novel proteins exhibit different toxicities, spectra of activity, and modes of action compared to conventional Bt proteins, thereby overcoming resistance that has developed against existing formulations.
2Adaptability or versatility
If a single insecticidal protein is used, then specific pest control is achieved, but broad spectrum efficacy is limited
Solution Approach 1:
The patent creates a universal platform of insecticidal proteins that can control multiple pest orders (Lepidoptera, Coleoptera, Diptera, Hymenoptera, Hemiptera) through a diversified portfolio of protein classes. Each protein class within this platform targets specific pest groups, but collectively they provide broad-spectrum coverage, allowing a single agricultural system to address diverse pest threats.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
TIC5290 proteins demonstrate significant inhibitory activity against target pests, reducing the likelihood of resistance development and providing effective pest control by targeting multiple pest species with distinct mechanisms of action.
Implementation Method 1
Upon ingestion by a susceptible insect, delta-endotoxins as well as secreted toxins exert their effects at the surface of the midgut epithelium, disrupting the cell membrane, leading to cell disruption and death.
Data Source
Figure 1
AI summary
A pesticidal protein class exhibiting toxic activity against Coleopteran, Lepidopteran, and Hemipteran pest species is disclosed, and includes, but is not limited to, TIC5290. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC5290 pesticidal protein. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran, Coleopteran and Hemipteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC5290 pesticidal protein of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, and Hemipteran species pests using the TIC5290 pesticidal protein are also provided.