Tiger Lily RNA Extraction for Cosmetic Safety
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Solution Overview
Problem
Current methods for extracting RNA from plant materials for cosmetic use are hindered by the use of toxic solvents like phenol and chloroform, which are not suitable for cosmetic applications, and existing protocols do not effectively provide a composition rich solely in RNA for addressing skin aging and photo-aging.
Innovation Solution
A topical composition enriched with low molecular weight RNA from Tiger lily (Lilium tigrinum) is developed, using an aqueous extraction process that avoids toxic solvents and includes a pH adjustment and filtration steps to obtain a physiologically acceptable medium, enhancing skin homeostasis and protection against aging.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If classic protocols using phenol and chloroform are used for RNA extraction, then extraction efficiency is improved, but toxicity of the product increases making it unsuitable for cosmetic use
Solution Approach 1:
The patent extracts and removes the toxic components (phenol, chloroform) from the extraction process, retaining only the beneficial RNA fraction. The method selectively isolates RNA while discarding harmful solvents, achieving both high extraction efficiency and cosmetic safety
Solution Approach 2:
The patent uses an intermediary purification step that separates RNA from toxic solvents. This intermediary process involves precipitation and filtration that acts as a mediator between the extraction phase and the final cosmetic product, eliminating harmful substances while preserving RNA activity
2Manufacturing precision
If multiple processing steps including detergents and solvents are used, then purification is improved, but product safety for cosmetic application deteriorates
Solution Approach 1:
The patent converts the harmful effect of extensive purification (which introduces toxic substances) into a benefit by using a purified form of the extraction method that achieves high purification without toxic residues. The RNA is purified through controlled precipitation and filtration that leaves no harmful traces
Solution Approach 2:
The patent changes the parameters of the purification process by controlling pH, temperature, and precipitation conditions to achieve high purification efficiency without requiring toxic solvents. The method uses physiological conditions that ensure both purity and cosmetic safety
3Quantity of substance
If existing extraction methods are used, then RNA extraction is achieved, but the composition contains DNA and other contaminants preventing effective anti-aging action
Solution Approach 1:
The patent segments the nucleic acid extraction process to separately isolate RNA from DNA and other contaminants. By using specific precipitation conditions and filtration steps, the method divides the complex extract into pure RNA fraction and discarded contaminants, achieving both quantity and purity requirements
Solution Approach 2:
The patent applies partial action by selectively precipitating only the RNA fraction under controlled conditions, leaving DNA and other substances in solution to be removed. This partial precipitation approach ensures high RNA purity while maintaining adequate RNA concentration for cosmetic effectiveness
Data Source
AI summary
The invention relates to a topical composition comprising an extract of Tiger lily (Lilium tigrinum) enriched in low molecular weight RNA. The invention also relates to a method of cosmetic care including the topical application a composition comprising an extract of Tiger lily (Lilium tigrinum) in a physiologically acceptable medium, in order to reduce skin signs of aging and photo-aging. The invention is also directed to a cosmetic method of treatment to improve cell viability, to improve cell protection against particulate matter and against DNA damages, and to reduce cellular senescence.