Tipburn-Resistant Lettuce Gene Locus Screening
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Solution Overview
Problem
Current lettuce plant cultivars lack sufficient resistance to tipburn, a physiological disorder caused by calcium deficiency, which leads to growth delays and reduced yields, especially in plant factories with artificial light where growth rates are high.
Innovation Solution
Identification of a tipburn-resistance gene locus on the 4th chromosome, specified by specific polynucleotide sequences, which can be used as a marker to breed tipburn-resistant lettuce plants.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If high illumination intensity and long lighting hours are used to increase growth rate, then productivity is improved, but tipburn occurs more frequently
Solution Approach 1:
The patent changes the genetic parameter of the lettuce plant by introducing a specific gene locus on the 4th chromosome that confers tipburn resistance. This allows the plant to tolerate high growth rates and illumination conditions without developing tipburn, thus resolving the contradiction between productivity and harmful factors through genetic modification rather than environmental adjustment
2Object-affected harmful factors
If growth rate is restrained by low illumination intensity and short lighting hours to prevent tipburn, then tipburn occurrence is reduced, but productivity decreases
Solution Approach 1:
The patent introduces a specific gene locus on the 4th chromosome that changes the plant's physiological response to calcium transport. This genetic parameter change enables the plant to maintain high productivity under normal illumination conditions without developing tipburn, eliminating the need to restrain growth rate
3Reliability
If conventional breeding methods are used to develop tipburn-resistant cultivars, then resistance is improved, but breeding time and complexity increase
Solution Approach 1:
The patent extracts and identifies the specific gene locus responsible for tipburn resistance on the 4th chromosome. By isolating this specific genetic element and using it as a marker, the complex breeding process is simplified into a targeted selection method, reducing both time and complexity while improving reliability
4Productivity
If tipburn-resistant lettuce plants are produced using molecular markers, then screening efficiency is improved, but measurement precision requirements increase
Solution Approach 1:
The patent changes the detection parameter by using a specific molecular marker (gene locus on the 4th chromosome) that shows clear polymorphism between resistant and susceptible plants. This parameter change enables efficient screening through standard molecular techniques while maintaining sufficient precision for practical breeding applications
Data Source
AI summary
Provided are a novel tipburn-resistance marker for lettuce plant, a tipburn-resistant lettuce plant, and a production method for the tipburn-resistant lettuce plant that uses the tipburn-resistance marker. Disclosed is a tipburn-resistant lettuce plant that includes a tipburn-resistance gene locus on the 4th chromosome, the tipburn-resistance gene locus being specified by at least one SNP marker selected from the group that consists of NC_056626.1-271576390, NC_056626.274188377, NC_056626.1-274188380, and NC_056626.274188382.