Tissue Cryopreservation Tool with Sealed Resin Bag
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Solution Overview
Problem
Existing tissue cryopreserving tools and methods face issues with long-term contact between containers and cooling media, leading to container deterioration and potential tissue scattering during thawing, as they require direct contact with liquid nitrogen for freezing, which can cause container damage and vapor pressure issues.
Innovation Solution
A collected living tissue cryopreserving tool comprising a thermally conductive tissue piece holder and a resin-made accommodating bag, where the tissue piece is immersed in a vitrification solution, placed on the holder, covered with a sheet, and sealed within the bag before contact with the cooling medium, preventing direct contact with liquid nitrogen and minimizing scattering risks.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Speed
If direct contact with liquid nitrogen is used for freezing, then rapid freezing and vitrification are achieved, but container deterioration and tissue scattering occur
Solution Approach 1:
The patent introduces an intermediary container system where the tissue is first placed in a sealed container, which is then placed in liquid nitrogen. This intermediary container prevents direct contact between the tissue/outer container and liquid nitrogen, avoiding deterioration while still enabling rapid freezing through thermal conduction from the container walls.
Solution Approach 2:
The tissue is preliminarily sealed in a container before exposure to liquid nitrogen. This preliminary sealing action protects the tissue from direct contact with liquid nitrogen and prevents scattering during the freezing process, while still allowing efficient heat transfer for rapid freezing.
2Temperature
If direct contact with liquid nitrogen is used for freezing, then vitrification is achieved, but vapor pressure and container damage occur
Solution Approach 1:
The patent uses an intermediary sealed container as a mediator between the tissue and liquid nitrogen. This container allows thermal energy transfer for achieving cryopreservation temperature while preventing direct exposure to liquid nitrogen vapor pressure, thus avoiding container damage from pressure stress.
3Reliability
If sealed container is used to prevent direct contact, then container deterioration is prevented, but freezing efficiency may be reduced
Solution Approach 1:
The patent employs thin-walled sealed containers that provide protection against liquid nitrogen contact while maintaining high thermal conductivity. The thin film structure minimizes thermal resistance, ensuring freezing efficiency is not significantly reduced despite the presence of the protective container.
Solution Approach 2:
The patent optimizes container parameters such as wall thickness, material thermal conductivity, and surface area to volume ratio. These parameter changes allow the container to provide adequate protection while maintaining sufficient heat transfer for efficient freezing, balancing reliability and productivity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid freezing of tissues without direct contact with cooling media, preventing container deterioration and tissue scattering, while maintaining effective cryopreservation through vitrification.
Implementation Method 1
a plate-shaped body part including a thermally conductive plate-shaped lower member (2)
Implementation Method 2
a collected tissue piece freezing method to be used in cryopreserving living cells such as mammalian ova
Data Source
Figure 1~2
Figure 3~4
Figure 5~6
AI summary
A collected living tissue cryopreserving tool (10) is composed of a tissue piece holder (1) and a resin-made accommodating bag (11). The tissue piece holder (1) is composed of a plate-shaped body part including a thermally conductive plate-shaped lower member (2) and a plate-shaped upper member (3) and a covering sheet (4) held by the plate-shaped body part. The tissue piece holder (1) has a tissue piece placing part (5) formed of a cut-out part (32) provided on the plate-shaped upper member (3) and a part, of an upper surface of the thermally conductive plate-shaped lower member (2), which is disposed at the cut-out part (32). The covering sheet (4) is capable of covering the tissue piece placing part (5).